Besnoitia besnoiti seroprevalence in beef, dairy and bullfighting cattle in Catalonia (north-eastern Spain): A cross-sectional study.

Garrido-Castañé, Ignasi; Romero, Anna Ortuño; Espuny, Joaquim Castellà; Hentrich, Brigitte; Basso, Walter Ubaldo (2019). Besnoitia besnoiti seroprevalence in beef, dairy and bullfighting cattle in Catalonia (north-eastern Spain): A cross-sectional study. Parasitology international, 69, pp. 71-74. Elsevier 10.1016/j.parint.2018.12.001

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Besnoitia besnoiti is the causative agent of bovine besnoitiosis, a chronic and debilitating disease of cattle that recently re-emerged and seems to be spreading in Europe. A cross-sectional serological study was carried out in different cattle herds in Catalonia, north-eastern Spain, to determine the seroprevalence of B. besnoiti in the region. A total of 791 serum samples (beef cattle n = 338, dairy cattle n = 291; bullfighting cattle n = 162) were tested. Sera were first screened for antibodies against Besnoitia using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) applying a cut-off that was lower than that recommended by the manufacturer in order to reach highest sensitivity. Sera above the chosen cut-off of 15% positivity (PP) were further tested by the Indirect Fluorescent Antibody Test (IFAT) and respectively positive results were confirmed by a B. besnoiti tachyzoite-based immunoblot. A total of 504/791 (63.7%) sera showed ELISA values above the selected cut-off, and 91 of these samples also yielded positive results in IFAT (cut-off titre 1:200). By immunoblot, a positive result was obtained in 93.4% (85 out of the 91) of the IFAT-positive samples. Interestingly, all confirmed Besnoitia-seropositive cases corresponded exclusively to beef cattle from the Pyrenees area, resulting in a prevalence of 25.1% (85/338) at the animal level and of 46% (36/78) at the herd level in this cattle group. No specific antibodies against Besnoitia could be detected in dairy and bullfighting cattle. The obtained results suggested that Besnoitia infections are present in Catalonia, consequently, diagnosis of this parasitic infection should be included in the sanitary control and before trading and movement of animals.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Hentrich, Brigitte and Basso, Walter Ubaldo

Subjects:

600 Technology > 630 Agriculture
500 Science > 590 Animals (Zoology)

ISSN:

1383-5769

Publisher:

Elsevier

Language:

English

Submitter:

Walter Ubaldo Basso

Date Deposited:

31 May 2019 13:39

Last Modified:

22 Oct 2019 20:28

Publisher DOI:

10.1016/j.parint.2018.12.001

PubMed ID:

30528534

Uncontrolled Keywords:

Beef Besnoitia Bullfighting Cattle Dairy Seroprevalence

BORIS DOI:

10.7892/boris.128794

URI:

https://boris.unibe.ch/id/eprint/128794

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