Effects of Single or Repeated Intranasal Administration of Umbilical Cord Stem Cells in Neonatal Rats with Hypoxic-Ischemic Brain Lesions

Oppliger, Byron; Jörger Messerli, Marianne; Müller, Martin; Reinhart, Ursula; Schneider, Philipp; Surbek, Daniel; Schoeberlein, Andreina (March 2017). Effects of Single or Repeated Intranasal Administration of Umbilical Cord Stem Cells in Neonatal Rats with Hypoxic-Ischemic Brain Lesions. Reproductive sciences, 24(Suppl 1), 300A-300A. Sage

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INTRODUCTION: Lately, there has been a significant increase in preterm-specific brain injuries that still remain an unresolved clinical issue. The majority of the infants born preterm with brain injuries develop non-cystic, diffuse white matter injury (WMI), characterized by an overall hypomyelination of the brain. Preterm brain injury is an important cause for long-term disability. To date, no cure has been found to treat such lesions. Intranasal delivery of Wharton’s jelly mesenchymal stem cells (WJ-MSC) might be the ideal, non-invasive therapeutic approach to restore the damaged brain. Therefore, our goal is to find an optimal treatment regimen of intranasally delivered WJ-MSC to achieve a maximum recovery after brain injury. METHODS: WJ-MSC (84’000 cells/ml) were delivered intranasally to Wistar rat pups that were previously brain-damaged (total 1*106 cells). Rat pups received either one, two or three treatments, at two days intervals. Animals were sacrificed 7 days after the first application of the cells. Fixed brains were analyzed by immunohistochemistry and real-time PCR. RESULTS: Treatment with WJ-MSC increased myelination and decreased astro- and microgliosis. Repeated intranasal delivery was not more effective than single treatment, as assessed by immunohistochemistry. However, multiple administrations increased significantly the expression of brain-derived neurotrophic factor (Bdnf) compared to single administration. CONCLUSIONS: In conclusion, intranasal delivery of WJ-MSC to the newborn after preterm brain damage has a neuroregenerative potential, which is probably mediated by a decreased astro- and microgliosis and an increased expression of important neurotrophic factors like Bdnf. Intranasal delivery of stem cells to the brain is an efficient and non-invasive method for stem cell treatment of perinatal brain damage. Financial support by Cryosave Switzerland, Mobiliar Jubiläumsstiftung, Switzerland and The Eagle Foundation, Switzerland.

Item Type:

Conference or Workshop Item (Poster)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Oppliger, Byron; Jörger Messerli, Marianne; Müller, Martin; Reinhart, Ursula; Schneider, Philipp; Surbek, Daniel and Schoeberlein, Andreina

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1933-7191

Publisher:

Sage

Funders:

[UNSPECIFIED] Cryo-Save Switzerland
[UNSPECIFIED] The Eagle Foundation, Crans-près-Céligny, Switzerland
[UNSPECIFIED] Mobiliar Jubiläumsstiftung, Switzerland

Language:

English

Submitter:

Andreina Schoeberlein

Date Deposited:

02 Oct 2019 16:32

Last Modified:

02 Oct 2019 16:32

BORIS DOI:

10.7892/boris.129491

URI:

https://boris.unibe.ch/id/eprint/129491

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