MAP kinase kinase 1 (MKK1) is essential for transmission of Trypanosoma brucei by Glossina morsitans

Morand, Sabine; Renggli, Christina Kunz; Roditi, Isabel; Vassella, Erik (2012). MAP kinase kinase 1 (MKK1) is essential for transmission of Trypanosoma brucei by Glossina morsitans. Molecular and biochemical parasitology, 186(1), pp. 73-6. Amsterdam: Elsevier 10.1016/j.molbiopara.2012.09.001

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MAP kinase kinase 1 (MKK1) is encoded by a single copy gene in Trypanosoma brucei. It has been shown recently that MKK1 is not essential for bloodstream forms [14]. To investigate the requirement for MKK1 in other life-cycle stages we generated null mutants in procyclic forms of a fly-transmissible strain. These grew normally in culture and were able to establish midgut infections in tsetse at normal rates and intensities, but were incapable of colonising the salivary glands. Transformation of null mutants with an ectopic copy of MKK1 enabled parasites to complete the life cycle in tsetse and infect mice. This is the first example of a gene that is indispensable for transmission of T. brucei. It also raises the possibility that activating the MKK1 signalling cascade in vitro might trigger the differentiation and proliferation of life-cycle stages of T. brucei that are currently refractory to culture.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Roditi, Isabel and Vassella, Erik

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:33

Last Modified:

06 Dec 2013 13:34

Publisher DOI:

10.1016/j.molbiopara.2012.09.001

PubMed ID:

22985893

Web of Science ID:

000311663300010

URI:

https://boris.unibe.ch/id/eprint/12979 (FactScience: 219478)

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