Distinct 3′ UTRs regulate the life-cycle-specific expression of two TCTP paralogs in Trypanosoma brucei

Jojic, Borka; Amodeo, Simona; Bregy, Irina; Ochsenreiter, Torsten (2018). Distinct 3′ UTRs regulate the life-cycle-specific expression of two TCTP paralogs in Trypanosoma brucei. Journal of cell biology, 131(9) Rockefeller Institute Press 10.1242/jcs.206417

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The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here, for the first time, we characterize the expression and function of TCTP in the human and animal pathogen, Trypanosoma brucei. We identified two paralogs (TCTP1 and TCTP2) that are differentially expressed in the life cycle of the parasite. The genes have identical 5′ untranslated regions (UTRs) and almost identical open-reading frames. The 3′UTRs differ substantially in sequence and length, and are sufficient for the exclusive expression of TCTP1 in procyclic- and TCTP2 in bloodstream-form parasites. Furthermore, we characterize which parts of the 3′UTR are needed for TCTP2 mRNA stability. RNAi experiments demonstrate that TCTP1 and TCTP2 expression is essential for normal cell growth in procyclic- and bloodstream-form parasites, respectively. Depletion of TCTP1 in the procyclic form cells leads to aberrant cell and mitochondrial organelle morphology, as well as enlarged, and a reduced number of, acidocalcisomes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Parasitologie
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Mitoch.
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Jojic, Borka; Amodeo, Simona; Bregy, Irina and Ochsenreiter, Torsten

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0021-9525

Publisher:

Rockefeller Institute Press

Funders:

[42] Schweizerischer Nationalfonds

Language:

English

Submitter:

Torsten Ochsenreiter

Date Deposited:

19 Sep 2019 11:18

Last Modified:

02 Nov 2019 14:06

Publisher DOI:

10.1242/jcs.206417

PubMed ID:

29661850

BORIS DOI:

10.7892/boris.129960

URI:

https://boris.unibe.ch/id/eprint/129960

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