RNA and Toll-Like Receptor 7 License the Generation of Superior Secondary Plasma Cells at Multiple Levels in a B Cell Intrinsic Fashion

Krüger, Caroline C.; Thoms, Franziska; Keller, Elsbeth; Leoratti, Fabiana M. S.; Vogel, Monique; Bachmann, Martin F. (2019). RNA and Toll-Like Receptor 7 License the Generation of Superior Secondary Plasma Cells at Multiple Levels in a B Cell Intrinsic Fashion. Frontiers in immunology, 10(736), p. 736. Frontiers Research Foundation 10.3389/fimmu.2019.00736

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Secondary plasma cells (PCs) originate from memory B cells and produce increased
levels of antibodies with higher affinity compared to PCs generated during primary
responses. Here we demonstrate that virus-like particles (VLPs) only induce secondary
PCs in the presence of toll-like receptor (TLR) 7 and if they are loaded with RNA.
Furthermore, adoptive transfer experiments demonstrate that RNA and TLR7 signaling
are required for secondary PC generation, both at the level ofmemory B cell as well as PC
differentiation. TLR7-signaling occurred in a B cell intrinsic manner as TLR7-deficient B
cells in an otherwise TLR7-competent environment failed to differentiate into secondary
PCs. Therefore, RNA inside VLPs is essential for the generation of memory B cells, which
are competent to differentiate to secondary PCs and for the differentiation of secondary
PCs themselves. While we have not tested all other TLR or non-TLR adjuvants with our
VLPs, these data have obvious implications for vaccine design, as RNA packaged into
VLPs is a simple way to enhance induction of memory B cells capable of generating
secondary PCs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology [discontinued]

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Krüger, Caroline Claire; De Souza Leoratti, Fabiana Maria; Vogel, Monique and Bachmann, Martin

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Caroline Claire Krüger

Date Deposited:

15 Nov 2019 15:34

Last Modified:

24 Nov 2019 02:40

Publisher DOI:

10.3389/fimmu.2019.00736

PubMed ID:

31024563

BORIS DOI:

10.7892/boris.130059

URI:

https://boris.unibe.ch/id/eprint/130059

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