Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia

Ebneter, Jacqueline A.; Heusser, Sally D.; Schraner, Elisabeth M.; Hehl, Adrian B.; Faso, Carmen (2016). Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia. Nature communications, 7(13859), p. 13859. Nature Publishing Group 10.1038/ncomms13859

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The genome of the protozoan parasite Giardia lamblia is organized in two diploid nuclei, which has so far precluded complete analysis of gene function. Here we use a previously developed Cre/loxP-based knock-out and selection marker salvage strategy in the human-derived isolate WB-C6 to eliminate all four copies of the Cyst-Wall-Protein-1 locus (CWP1). Because these loci are silenced in proliferating trophozoites and highly expressed only in encysting cells, CWP1 ablation allows functional characterization of a conditional phenotype in parasites induced to encyst. We show that encysting Δcwp1 cells are unable to establish the stage-regulated trafficking machinery with Golgi-like encystation-specific vesicles required for cyst-wall formation but show morphological hallmarks of cyst development and karyokinesis. This ‘pseudocyst’ phenotype is rescued by transfection of Δcwp1 cells with an episomally maintained CWP1 expression vector. Genome editing in genera Giardia and Trypanosoma are the only reported examples addressing questions on pathogen transmission within the Excavata supergroup.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Parasitologie

UniBE Contributor:

Faso, Carmen

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Carmen Faso

Date Deposited:

27 Sep 2019 11:19

Last Modified:

23 Oct 2019 21:52

Publisher DOI:

10.1038/ncomms13859

PubMed ID:

27976675

BORIS DOI:

10.7892/boris.131608

URI:

https://boris.unibe.ch/id/eprint/131608

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