Compatibility of 5-ethynyl-2’F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

Levi-Acobas, Fabienne; Katolik, Adam; Röthlisberger, Pascal; Cokelaer, Thomas; Sarac, Ivo; Damha, Masad J.; Leumann, Christian J.; Hollenstein, Marcel (2019). Compatibility of 5-ethynyl-2’F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers. Organic & biomolecular chemistry, 17(35), pp. 8083-8087. Royal Society of Chemistry 10.1039/C9OB01515A

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A modified nucleoside triphosphate bearing two modifications based on a 2’-deoxy-2’-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2’F-ANA UTP) was synthesized. This nucleotide analog was shown to be compatible with enzymatic XZA synthesis and with reverse transcription of XZA to DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Katolik, Adam and Leumann, Christian

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
500 Science

ISSN:

1477-0520

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Christian Leumann

Date Deposited:

29 Aug 2019 15:52

Last Modified:

23 Oct 2019 08:26

Publisher DOI:

10.1039/C9OB01515A

BORIS DOI:

10.7892/boris.132804

URI:

https://boris.unibe.ch/id/eprint/132804

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