Levi-Acobas, Fabienne; Katolik, Adam; Röthlisberger, Pascal; Cokelaer, Thomas; Sarac, Ivo; Damha, Masad J.; Leumann, Christian J.; Hollenstein, Marcel (2019). Compatibility of 5-ethynyl-2’F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers. Organic & biomolecular chemistry, 17(35), pp. 8083-8087. Royal Society of Chemistry 10.1039/C9OB01515A
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A modified nucleoside triphosphate bearing two modifications based on a 2’-deoxy-2’-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2’F-ANA UTP) was synthesized. This nucleotide analog was shown to be compatible with enzymatic XZA synthesis and with reverse transcription of XZA to DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Katolik, Adam, Leumann, Christian |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry 500 Science |
ISSN: |
1477-0520 |
Publisher: |
Royal Society of Chemistry |
Language: |
English |
Submitter: |
Christian Leumann |
Date Deposited: |
29 Aug 2019 15:52 |
Last Modified: |
05 Dec 2022 15:30 |
Publisher DOI: |
10.1039/C9OB01515A |
BORIS DOI: |
10.7892/boris.132804 |
URI: |
https://boris.unibe.ch/id/eprint/132804 |
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