Proteomic changes in the rat brain induced by homogenous irradiation and by the bystander effect resulting from high energy synchrotron X-ray microbeams

Smith, Richard W; Wang, Jiaxi; Schültke, Elisabeth; Seymour, Colin B; Bräuer-Krisch, Elke; Laissue, Jean A; Blattmann, Hans; Mothersill, Carmel E (2013). Proteomic changes in the rat brain induced by homogenous irradiation and by the bystander effect resulting from high energy synchrotron X-ray microbeams. International journal of radiation biology, 89(2), pp. 118-27. London: Taylor & Francis 10.3109/09553002.2013.732252

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Abstract Purpose: To further evaluate the use of microbeam irradiation (MBI) as a potential means of non-invasive brain tumor treatment by investigating the induction of a bystander effect in non-irradiated tissue. Methods: Adult rats were irradiated with 35 or 350 Gy at the European Synchotron Research Facility (ESRF), using homogenous (broad beam) irradiation (HI) or a high energy microbeam delivered to the right brain hemisphere only. The proteome of the frontal lobes were then analyzed using two-dimensional electrophoresis (2-DE) and mass spectrometry. Results: HI resulted in proteomic responses indicative of tumourigenesis; increased albumin, aconitase and triosphosphate isomerase (TPI), and decreased dihydrolipoyldehydrogenase (DLD). The MBI bystander effect proteomic changes were indicative of reactive oxygen species mediated apoptosis; reduced TPI, prohibitin and tubulin and increased glial fibrillary acidic protein (GFAP). These potentially anti-tumourigenic apoptotic proteomic changes are also associated with neurodegeneration. However the bystander effect also increased heat shock protein (HSP) 71 turnover. HSP 71 is known to protect against all of the neurological disorders characterized by the bystander effect proteome changes. Conclusions: These results indicate that the collective interaction of these MBI-induced bystander effect proteins and their mediation by HSP 71, may confer a protective effect which now warrants additional experimental attention.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Laissue, Jean

ISSN:

0955-3002

Publisher:

Taylor & Francis

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:34

Last Modified:

15 Nov 2016 07:08

Publisher DOI:

10.3109/09553002.2013.732252

PubMed ID:

23004567

Web of Science ID:

000314446700006

URI:

https://boris.unibe.ch/id/eprint/13290 (FactScience: 219805)

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