Changes in gene expression of intervertebral discs from diffuse idiopathic skeletal hyperostosis (DISH) patients compared to traumatic/degenerative intervertebral discs

May, Rahel D.; Frauchiger, Daniela A.; Albers, Christoph E.; Benneker, Lorin M.; Gantenbein, Benjamin (2019). Changes in gene expression of intervertebral discs from diffuse idiopathic skeletal hyperostosis (DISH) patients compared to traumatic/degenerative intervertebral discs. In: 7th International Congress on Biotechnologies for Spinal Surgery (BioSpine7). Rom, Italy. 3-5 April.

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Introduction: Diffuse idiopathic skeletal hyperostosis (DISH) is characterized by the formation of bone along the anterior spine. Further ossification of the outer intervertebral discs (IVD) can be observed. However, the nucleus pulposus (NP) remains unaffected. We investigated the phenotype of disc cells of DISH patients and why the IVD resists ossification. Methods: Cells were isolated from IVD tissue of DISH and control patients. TGFβ BMP signaling pathway genes were compared by qPCR. IVDs of three DISH patients were tested against three control patients (same disc level and similar age). IVD of two donors could be separated in NP, annulus fibrosus (AF) and cartilaginous endplate (CEP), one donor was investigated without discriminated IVD tissue. Results: In six of the seven comparisons a mean up-regulation of Interleukin 6 (IL-6) was detected (mean ± SEM of all comparisons: 88.8 ± 79.4-fold in DISH-IVD compared to controls). Early Growth Response 2 (EGR2) and Insulin-like Growth Factor 1 (IGF1) up-regulated in DISH-IVD donors (i.e., 20.5 ± 12.4-fold and 19.0 ± 19.5-fold, respectively). The two Growth and Differentiation Factors 5 and 6 (GDF5 and 6) were downregulated in two of the three DISH-IVDs (i.e., -21.9 ± 16.2-fold and -8.2 ± 4.2-fold, respectively). Conclusion: Most interestingly, the DISH-IVD cells showed a considerable change in IGF1 and IL-6. IGF1 was already determined as a serum marker for rheumatic diseases, such as DISH. These results are unexpected considering the fact that the ossification occurs in the neighboring ligaments and enthesis leaving the inner part of the IVD macroscopically unaffected.

Item Type:

Conference or Workshop Item (Paper)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

May, Rahel Deborah; Frauchiger, Daniela Angelika; Albers, Christoph; Benneker, Lorin Michael and Gantenbein, Benjamin

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

04 Sep 2019 13:21

Last Modified:

23 Oct 2019 12:45

Additional Information:

Proceedings of the 7th International Congress on Biotechnologies for Spinal Surgery (BioSpine7)

BORIS DOI:

10.7892/boris.132960

URI:

https://boris.unibe.ch/id/eprint/132960

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