Stimulation of intervertebral disc cells in alginate bead culture with bone morphogenetic protein 2 and/or L51P

May, Rahel Deborah; Frauchiger, D.A.; Albers, Christoph E.; Benneker, Lorin M.; Hofstetter, Wilhelm; Gantenbein, Benjamin (2019). Stimulation of intervertebral disc cells in alginate bead culture with bone morphogenetic protein 2 and/or L51P. In: TERMIS EU chapter meeting. Rhodes, Greece. 27th-31s tMay.

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INTRODUCTION: In clinics, Bone Morphogenetic Protein 2 (BMP2) was applied for the support during spinal fusion. Further BMP2 was tested in IVD models and showed potential for IVD regeneration. The aim of this study is the investigation of BMP2 and the BMP2 analogue, L51P, on different cell types of the human IVD in 3D alginate beads, particularly their plasticity to undergo bone formation. METHODS: Human nucleus pulposus (NPC), annulus fibrosus (AFC) and cartilaginous endplate cells (CEPC) were each encapsulated in 1.2% alginate at a density of 4 Mio/mL. NPC, AFC, and CEPC beads were then cultured in α-MEM or osteogenic medium (OM) supplemented with 10% FBS and 100 ng/mL BMP2 and/or L51P for 21 days. Medium supplemented with cytokines was refreshed twice per week. Beads were snap frozen with liquid nitrogen after 7 days for mRNA analysis of Aggrecan (ACAN), Collagen type1 (COL1), Collagen type 2 (COL2) and runt-related transcription factor 2 (RUNX2) qPCR. Further, beads were stained with Alcian Blue after 21 days. RESULTS & DISCUSSION: ACAN expression was the highest up-regulated in IVD cells stimulated with OM and 100 ng/mL BMP2 and L51P compared to the negative control (basal medium only) in NPC, AFC and CEPC (mean ± SEM NP: 18.95 ± 15.65). The same was true for COL2 expression (NP: 72.47 ± 62.95). COL1 remained unaffected (N=2). CONCLUSIONS: In this study, we showed the trend of an increase in ACAN and COL2 gene expression in stimulated cells. Interestingly the co-treatment of BMP2 and L51P showed a cumulative effect towards an increased ECM production.

Item Type:

Conference or Workshop Item (Abstract)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
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04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB [discontinued]
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

May, Rahel Deborah; Albers, Christoph; Benneker, Lorin Michael; Hofstetter, Wilhelm and Gantenbein, Benjamin

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

04 Sep 2019 14:22

Last Modified:

03 Nov 2019 05:26

Additional Information:

eCM Periodical, TERMIS EU Abstracts

BORIS DOI:

10.7892/boris.132970

URI:

https://boris.unibe.ch/id/eprint/132970

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