Macropinocytosis contributes to hantavirus entry into human airway epithelial cells.

Torriani, Giulia; Mayor, Jennifer; Zimmer, Gert; Kunz, Stefan; Rothenberger, Sylvia; Engler, Olivier (2019). Macropinocytosis contributes to hantavirus entry into human airway epithelial cells. Virology, 531, pp. 57-68. Elsevier 10.1016/j.virol.2019.02.013

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Hantaviruses are emerging rodent-borne negative-strand RNA viruses associated with severe human diseases. Zoonotic transmission occurs via aerosols of contaminated rodent excreta and cells of the human respiratory epithelium represent likely early targets. Here we investigated cellular factors involved in entry of the pathogenic Old and New World hantaviruses Hantaan virus (HTNV) and Andes virus (ANDV) into human respiratory epithelial cells. Screening of a kinase inhibitor library using a biocontained recombinant vesicular stomatitis virus pseudotype platform revealed differential requirement for host kinases for HTNV and ANDV entry and provided first hints for an involvement of macropinocytosis. Examination of a selected panel of well-defined inhibitors of endocytosis confirmed that both HTNV and ANDV enter human respiratory epithelial cells via a pathway that critically depends on sodium proton exchangers and actin, hallmarks of macropinocytosis. However, HTNV and ANDV differed in their individual requirements for regulatory factors of macropinocytosis, indicating virus-specific differences.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Zimmer, Gert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1096-0341

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

04 Sep 2019 17:08

Last Modified:

26 Oct 2019 06:58

Publisher DOI:

10.1016/j.virol.2019.02.013

PubMed ID:

30852272

Uncontrolled Keywords:

Endocytosis Hantavirus Macropinocytosis Signaling Tropism Viral entry

BORIS DOI:

10.7892/boris.132994

URI:

https://boris.unibe.ch/id/eprint/132994

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