Determining the Replication Kinetics and Cellular Tropism of Influenza D Virus on Primary Well-Differentiated Human Airway Epithelial Cells.

Holwerda, Melle; Kelly, Jenna; Laloli, Laura; Stürmer, Isabel; Portmann, Jasmine; Stalder, Hanspeter; Dijkman, Ronald (2019). Determining the Replication Kinetics and Cellular Tropism of Influenza D Virus on Primary Well-Differentiated Human Airway Epithelial Cells. Viruses, 11(4) Molecular Diversity Preservation International MDPI 10.3390/v11040377

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Influenza viruses are notorious pathogens that frequently cross the species barrier with often severe consequences for both animal and human health. In 2011, a novel member of the Orthomyxoviridae family, Influenza D virus (IDV), was identified in the respiratory tract of swine. Epidemiological surveys revealed that IDV is distributed worldwide among livestock and that IDV-directed antibodies are detected in humans with occupational exposure to livestock. To identify the transmission capability of IDV to humans, we determined the viral replication kinetics and cell tropism using an in vitro respiratory epithelium model of humans. The inoculation of IDV revealed efficient replication kinetics and apical progeny virus release at different body temperatures. Intriguingly, the replication characteristics of IDV revealed higher replication kinetics compared to Influenza C virus, despite sharing the cell tropism preference for ciliated cells. Collectively, these results might indicate why IDV-directed antibodies are detected among humans with occupational exposure to livestock.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Holwerda, Melle; Kelly, Jenna Nicole; Laloli, Laura; Stürmer, Isabel; Portmann, Jasmine; Stalder, Hanspeter and Dijkman, Ronald

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1999-4915

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

04 Sep 2019 17:27

Last Modified:

26 Oct 2019 07:47

Publisher DOI:

10.3390/v11040377

PubMed ID:

31022887

Uncontrolled Keywords:

human respiratory epithelium influenza D virus zoonosis

BORIS DOI:

10.7892/boris.132999

URI:

https://boris.unibe.ch/id/eprint/132999

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