The highly diverged trypanosomal MICOS complex is organized in a non‐essential integral membrane and an essential peripheral module

Eichenberger, Claudia; Oeljeklaus, Silke; Bruggisser, Julia Maria; Mani, Jan; Haenni, Beat; Kaurov, Iosif; Niemann, Moritz; Zuber, Benoît; Lukeš, Julius; Hashimi, Hassan; Warscheid, Bettina; Schimanski, Bernd; Schneider, André (2019). The highly diverged trypanosomal MICOS complex is organized in a non‐essential integral membrane and an essential peripheral module. Molecular microbiology, 112(6), pp. 1731-1743. Wiley 10.1111/mmi.14389

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The mitochondrial contact site and cristae organization system (MICOS) mediates the formation of cristae, invaginations in the mitochondrial inner membrane. The highly diverged MICOS complex of the parasitic protist Trypanosoma brucei consists of 9 subunits. Except for two Mic10-like and a Mic60-like protein, all subunits are specific for kinetoplastids. Here we determined on a proteome-wide scale how ablation of individual MICOS subunits affects the levels of the other subunits. The results reveal co-regulation of TbMic10-1, TbMic10-2, TbMic16 and TbMic60, suggesting that these non-essential, integral inner membrane proteins form an interdependent network. Moreover, the ablation of TbMic34 and TbMic32 reveals another network consisting of the essential, intermembrane space-localized TbMic20, TbMic32, TbMic34 and TbMic40, all of which are peripherally associated with the inner membrane. The downregulation of TbMic20, TbMic32 and TbMic34 also interferes with mitochondrial protein import and reduces the size of the TbMic10-containing complexes. Thus, the diverged MICOS of trypanosomes contains two subcomplexes: a non-essential membrane-integrated one, organized around the conserved Mic10 and Mic60, that mediates cristae formation, and an essential membrane-peripheral one consisting of four kinetoplastid-specific subunits, that is required for import of intermembrane space proteins.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Eichenberger, Claudia, Bruggisser, Julia Maria, Mani, Jan, Niemann, Moritz, Zuber, Benoît, Schimanski, Bernd, Schneider, André


600 Technology > 610 Medicine & health
500 Science > 540 Chemistry
500 Science > 570 Life sciences; biology








Christina Schüpbach

Date Deposited:

25 Sep 2019 13:53

Last Modified:

05 Dec 2022 15:30

Publisher DOI:


PubMed ID:





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