Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children

Mégevand, C; Gervaix, A; Heininger, U; Berger, C; Aebi, C; Vaudaux, B; Kind, C; Gnehm, H-P; Hitzler, M; Renzi, G; Schrenzel, J; François, P (2010). Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children. Clinical microbiology and infection, 16(9), pp. 1414-20. Oxford: Blackwell Publishing 10.1111/j.1469-0691.2009.03090.x

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Nasal carriage of Staphylococcus aureus contributes to an increased risk of developing an infection with the same bacterial strain. Genetic regulatory elements and toxin-expressing genes are virulence factors associated with the pathogenic potential of S. aureus. We undertook an extensive molecular characterization of methicillin-susceptible S. aureus (MSSA) carried by children. MSSA were recovered from the nostrils of children. The presence of Panton-Valentine leukocidin (PVL), exfoliatins A and B (exfoA and exfoB), and the toxic-shock staphylococcal toxin (TSST-1) and agr group typing were determined by quantitative PCR. A multiple-locus variable-number of tandem repeat analysis (MLVA) assay was also performed for genotyping. Five hundred and seventy-two strains of MSSA were analysed. Overall, 30% were positive for toxin-expressing genes: 29% contained one toxin and 1.6% two toxins. The most commonly detected toxin gene was tst, which was present in 145 (25%) strains. The TSST-1 gene was significantly associated with the agr group 3 (OR 56.8, 95% CI 32.0-100.8). MLVA analysis revealed a large diversity of genetic content and no clonal relationship was demonstrated among the analysed MSSA strains. Multilocus sequence typing confirmed this observation of diversity and identified ST45 as a frequent colonizer. This broad diversity in MSSA carriage strains suggests a limited selection pressure in our geographical area.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
UniBE Contributor:	Aebi, Christoph, Schrenzel, Jacques
ISSN:	1198-743X
Publisher:	Blackwell Publishing
Language:	English
Submitter:	Anette van Dorland
Date Deposited:	04 Oct 2013 14:07
Last Modified:	05 Dec 2022 14:00
Publisher DOI:	10.1111/j.1469-0691.2009.03090.x
PubMed ID:	19845693
Web of Science ID:	000281310900019
URI:	https://boris.unibe.ch/id/eprint/134 (FactScience: 196467)