Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children

Mégevand, C; Gervaix, A; Heininger, U; Berger, C; Aebi, C; Vaudaux, B; Kind, C; Gnehm, H-P; Hitzler, M; Renzi, G; Schrenzel, J; François, P (2010). Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children. Clinical microbiology and infection, 16(9), pp. 1414-20. Oxford: Blackwell Publishing 10.1111/j.1469-0691.2009.03090.x

Full text not available from this repository. (Request a copy)

Nasal carriage of Staphylococcus aureus contributes to an increased risk of developing an infection with the same bacterial strain. Genetic regulatory elements and toxin-expressing genes are virulence factors associated with the pathogenic potential of S. aureus. We undertook an extensive molecular characterization of methicillin-susceptible S. aureus (MSSA) carried by children. MSSA were recovered from the nostrils of children. The presence of Panton-Valentine leukocidin (PVL), exfoliatins A and B (exfoA and exfoB), and the toxic-shock staphylococcal toxin (TSST-1) and agr group typing were determined by quantitative PCR. A multiple-locus variable-number of tandem repeat analysis (MLVA) assay was also performed for genotyping. Five hundred and seventy-two strains of MSSA were analysed. Overall, 30% were positive for toxin-expressing genes: 29% contained one toxin and 1.6% two toxins. The most commonly detected toxin gene was tst, which was present in 145 (25%) strains. The TSST-1 gene was significantly associated with the agr group 3 (OR 56.8, 95% CI 32.0-100.8). MLVA analysis revealed a large diversity of genetic content and no clonal relationship was demonstrated among the analysed MSSA strains. Multilocus sequence typing confirmed this observation of diversity and identified ST45 as a frequent colonizer. This broad diversity in MSSA carriage strains suggests a limited selection pressure in our geographical area.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research

UniBE Contributor:

Aebi, Christoph and Schrenzel, Jacques

ISSN:

1198-743X

Publisher:

Blackwell Publishing

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:07

Last Modified:

17 Mar 2015 19:07

Publisher DOI:

10.1111/j.1469-0691.2009.03090.x

PubMed ID:

19845693

Web of Science ID:

000281310900019

URI:

https://boris.unibe.ch/id/eprint/134 (FactScience: 196467)

Actions (login required)

Edit item Edit item
Provide Feedback