Polymer-coated nanoparticles and their effects on mitochondrial function in brain endothelial cells.

Bittner, Aniela; Ducray, Angélique Dominique; Stoffel, Michael Hubert; Felser, Andrea; Mevissen, Meike (2019). Polymer-coated nanoparticles and their effects on mitochondrial function in brain endothelial cells. Toxicology and Applied Pharmacology, 385, p. 114800. Elsevier 10.1016/j.taap.2019.114800

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Laser tissue soldering is a novel treatment method for injuries of hollow organs such as cerebrovascular aneurysms. Nanomaterials contained in the solder are foreign to the body. Hence, it is indispensable to carefully examine possible adverse effects prior to introducing this technique. The aim of this study was to characterize the impact of different concentrations of polymer-coated silica nanoparticles (NPs) on mitochondrial function and integrity of brain endothelial cells using the rat brain capillary endothelial cell line rBCEC4. At maximal capacity, NP exposure resulted in a decrease in the oxygen consumption rate whereas glycolysis was not affected. In combination with a stressor, i.e. lack of glucose in the medium, NP exposure interfered primarily with glycolytic ATP generation rather than oxidative phosphorylation. Furthermore, NPs caused a metabolic shift towards a stressed phenotype, exhibiting increased levels of the oxygen consumption rate and the extracellular acidification rate compared to untreated controls. Overall, mitochondrial mass, distribution and morphology as well as intracellular ATP content were not altered. The mitochondrial membrane potential was increased after exposure to the highest NP concentration and the content of proteins involved in mitochondrial dynamics was changed slightly, indicating possible modifications of the fusion / fission balance. In conclusion, PCL-NP exposure changed mitochondrial respiration, especially under glucose deprivation, but did not affect mitochondrial morphology and distribution. Further studies are needed to investigate whether the functional effects are transient or long-term as this will be crucial for the use of these NPs in laser tissue soldering.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Bittner, Aniela; Ducray, Angélique; Stoffel, Michael Hubert; Felser, Andrea Debora and Mevissen, Meike

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0041-008X

Publisher:

Elsevier

Language:

English

Submitter:

Vanessa Alice Blum

Date Deposited:

19 Nov 2019 16:14

Last Modified:

07 Jan 2020 10:23

Publisher DOI:

10.1016/j.taap.2019.114800

PubMed ID:

31678605

Uncontrolled Keywords:

Laser tissue soldering Mitochondrial respiration Nanomedicine rBCEC4 Cells

BORIS DOI:

10.7892/boris.135049

URI:

https://boris.unibe.ch/id/eprint/135049

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