Andrés-Delgado, Laura; Ernst, Alexander; Galardi-Castilla, María; Bazaga, David; Peralta, Marina; Münch, Juliane; González-Rosa, Juan M.; Marques, Inês; Tessadori, Federico; de la Pompa, José Luis; Vermot, Julien; Mercader, Nadia (2019). Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells. Development, 146(13) Company of Biologists Limited 10.1242/dev.174961
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The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Ernst, Alexander Uwe Johann, dos Santos Marques, Ines Joao, Mercader Huber, Nadia Isabel |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0950-1991 |
Publisher: |
Company of Biologists Limited |
Language: |
English |
Submitter: |
Nadia Isabel Mercader Huber |
Date Deposited: |
20 Nov 2019 11:21 |
Last Modified: |
05 Dec 2022 15:32 |
Publisher DOI: |
10.1242/dev.174961 |
PubMed ID: |
31175121 |
BORIS DOI: |
10.7892/boris.135136 |
URI: |
https://boris.unibe.ch/id/eprint/135136 |
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