Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells

Andrés-Delgado, Laura; Ernst, Alexander; Galardi-Castilla, María; Bazaga, David; Peralta, Marina; Münch, Juliane; González-Rosa, Juan M.; Marques, Inês; Tessadori, Federico; de la Pompa, José Luis; Vermot, Julien; Mercader, Nadia (2019). Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells. Development, 146(13) Company of Biologists Limited 10.1242/dev.174961

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The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Faculty Institutions > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ernst, Alexander Uwe Johann; dos Santos Marques, Ines Joao and Mercader Huber, Nadia Isabel

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

20 Nov 2019 11:21

Last Modified:

20 Nov 2019 13:34

Publisher DOI:

10.1242/dev.174961

PubMed ID:

31175121

BORIS DOI:

10.7892/boris.135136

URI:

https://boris.unibe.ch/id/eprint/135136

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