Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears

Hewer, E.; Schmitt, A. M. (2020). Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears. Acta cytologica, 64(4), pp. 375-377. Karger 10.1159/000505254

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Rapid on-site evaluation (ROSE) is one of cytopathology's "unique selling propositions." The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Cytopathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Endocrinology, Diabetology and Clinical Nutrition

UniBE Contributor:

Hewer, Ekkehard and Schmitt Kurrer, Anja

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0001-5547

Publisher:

Karger

Language:

English

Submitter:

Ekkehard Hewer

Date Deposited:

11 Feb 2020 09:26

Last Modified:

14 Jul 2020 01:31

Publisher DOI:

10.1159/000505254

PubMed ID:

31962321

BORIS DOI:

10.7892/boris.139380

URI:

https://boris.unibe.ch/id/eprint/139380

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