Detection of specific Treponema species and Dichelobacter nodosus from digital dermatitis (Mortellaro's disease) lesions in Swiss cattle.

Alsaaod, Maher; Locher, Iwan; Jores, Jörg; Grimm, Pauline; Brodard, Isabelle; Steiner, Adrian; Kuhnert, Peter (2019). Detection of specific Treponema species and Dichelobacter nodosus from digital dermatitis (Mortellaro's disease) lesions in Swiss cattle. Schweizer Archiv für Tierheilkunde, 161(4), pp. 207-215. Gesellschaft Schweizer Tierärztinnen und Tierärzte 10.17236/sat00201

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INTRODUCTION The aim of this study was to determine the prevalence of the three Treponema species as well as D. nodosus in Digital dermatitis (DD) and slurry of Swiss cattle using PCR. A total of 86 specimens from 24 farms were enrolled in the study. Slurry samples from 21 DD-affected and one unaffected farm were collected to assess the potential of environmental transmission. Nested and real-time PCR were performed from the specimens to detect Treponema species and D. nodosus, respectively. The DD-stages were positive for at least one or more of the DD-associated Treponema species in 50 of 61 cases (82.0%) and in 9 of 25 cases (36.0%) in unaffected animals. Infected animals with small focal active lesions showed a significantly lower prevalence (14.8%) compared to the other DD stages (67.2%; P=0.011). Most prevalent was T. phagedenis (65.1%). D. nodosus was detected in 51.8% of clinical DD lesions and 24.1% in unaffected cases, but its presence was not significantly associated with the various DD-stages. All samples positive for D. nodosus contained the acid protease gene aprB2 but were negative for aprV2, the latter associated with virulence in sheep foot rot. Control farms were negative for all DD-associated Treponema species while positive for aprB2 and negative for aprV2. The presence of aprB2 suggests it is ubiquitous in the animal environment. With respect to the slurry samples, three out of 21 specimens (14.3%) were positive for one or more of the DD-associated Treponema species and eleven out of 21 specimens (52.4%) were positive for aprB2 and negative for aprV2 of D. nodosus. In conclusion, an association was found between the presence of clinical DD and specific Treponema species, while for D. nodosus no such link with DD lesions could be observed.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Alsaaod, Maher; Locher, Iwan; Jores, Jörg; Grimm, Pauline; Brodard, Isabelle; Steiner, Adrian and Kuhnert, Peter

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1664-2848

Publisher:

Gesellschaft Schweizer Tierärztinnen und Tierärzte

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

14 Feb 2020 07:51

Last Modified:

13 Mar 2020 15:49

Publisher DOI:

10.17236/sat00201

PubMed ID:

30942188

Uncontrolled Keywords:

Dichelobacter nodosus, Digital Dermatitis, Klauen, Lahmheit, PCR, Rind, Treponema spp.

BORIS DOI:

10.7892/boris.139742

URI:

https://boris.unibe.ch/id/eprint/139742

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