First molecular detection of ball python nidovirus in Italy - Short communication.

Rampacci, Elisa; Masi, Marco; Origgi, Francesco Carlo; Stefanetti, Valentina; Bottinelli, Marco; Selleri, Paolo; Coletti, Mauro; Passamonti, Fabrizio (2019). First molecular detection of ball python nidovirus in Italy - Short communication. Acta veterinaria Hungarica, 67(1), pp. 127-134. Akadémiai Kiadó 10.1556/004.2019.014

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A retrospective study was conducted to investigate the presence of ferlavirus, ball python nidovirus and bacteria in 32 tracheobronchial lavages from ball pythons raised in captivity and affected by respiratory disease. A touchdown reverse transcription polymerase reaction (RT-PCR) was performed to detect ball python nidovirus RNA targeting a 260-bp portion of the ORF1a gene, while a nested RT-PCR was applied to identify RNA targeting the 518-bp ferlavirus partial L gene. RT-PCR positive products were submitted for Sanger's sequencing and phylogeny reconstruction. Bacteriological examinations were performed to diagnose a possible bacterial involvement. BLAST analysis revealed that the nucleotide sequences of the six (18.8%) RT-PCR positive amplicons were 90-97% identical to the partial sequence of the ORF1a gene of the recently described ball python nidovirus. All tested snakes were negative for ferlavirus. Thirteen out of 32 samples (40.6%) were bacteriologically positive. Respiratory tract diseases can be a substantial problem for snake breeders, considering the rapid transmission of respiratory pathogens. The results and published studies show that ball python nidovirus is circulating in python collections and could be linked to suboptimal management practices. Surveillance programs are desirable as part of the routine snake health assessment. Tracheobronchial lavage is a fast, practical, cost-effective procedure for sample collection.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Origgi, Francesco

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0236-6290

Publisher:

Akadémiai Kiadó

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

31 Mar 2020 09:19

Last Modified:

31 Mar 2020 09:19

Publisher DOI:

10.1556/004.2019.014

PubMed ID:

30922084

Uncontrolled Keywords:

Ball python nidovirus RT-PCR ferlavirus phylogeny reconstruction respiratory disease

BORIS DOI:

10.7892/boris.141723

URI:

https://boris.unibe.ch/id/eprint/141723

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