Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba

Jerez Puebla, Luis Enrique; Núñez Fernández, Fidel A.; Fraga, Jorge; Rivero, Lázara Rojas; Millán, Iraís Atencio; Valdés, Lucía Ayllón; Silva, Isabel Martínez; Müller, Norbert; Robertson, Lucy J. (2020). Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba. Experimental parasitology, 209, p. 107814. Elsevier 10.1016/j.exppara.2019.107814

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Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in both humans and other animals. However, consensus on the most appropriate genotyping scheme for optimal characterization of G. duodenalis isolates is lacking. In this article we present the results of three descriptive observational studies conducted in Havana, Cuba between 2010 and 2013, with the aim of comparing the results from molecular (PCR) approaches targeting different genes in order to assign with confidence 224 isolates of G. duodenalis to the correct assemblages. In each sub-study, following DNA isolation by the phenol/chloroform/isoamyl alcohol extraction method, PCR targeting the triose phosphate isomerase (tpi) gene was used for molecular characterization, as well as one additional PCR-method targeting another gene or pair of genes. DNA amplification was obtained in 87%, 83%, and 80% in the three sub-studies. Although excellent agreement (kappa index = 1) was recorded between results from some pairs of genes, for other combinations only moderate or substantial agreement was achieved. These results highlight the importance of interpretation of genotyping data, especially when single genetic markers are used. From the results of our studies, PCR targeting a combination of the tpi gene and the intergenic spacer region of rDNA may be a useful approach for the molecular characterization of G. duodenalis isolates.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Müller, Norbert

Subjects:

600 Technology > 630 Agriculture
500 Science
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

0014-4894

Publisher:

Elsevier

Language:

English

Submitter:

Norbert Müller

Date Deposited:

31 Mar 2020 15:43

Last Modified:

05 Apr 2020 02:49

Publisher DOI:

10.1016/j.exppara.2019.107814

PubMed ID:

31816280

BORIS DOI:

10.7892/boris.141927

URI:

https://boris.unibe.ch/id/eprint/141927

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