Not every sperm counts: Male fertility in solitary bees, Osmia cornuta

Strobl, Verena; Straub, Lars; Bruckner, Selina; Albrecht, Matthias; Maitip, Jakkrawut; Kolari, Eleonora; Chantawannakul, Panuwan; Williams, Geoffrey R.; Neumann, Peter (2019). Not every sperm counts: Male fertility in solitary bees, Osmia cornuta. PLoS ONE, 14(3), e0214597. Public Library of Science 10.1371/journal.pone.0214597

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Reproductive strategies can act as strong selective forces on reproductive traits of male insects, resulting in species-specific variation in sperm quantity and viability. For solitary bees, basic measures of sperm quantity and viability are scarce. Here we evaluated for the first time quantity and viability of sperm in male Osmia cornuta solitary bees at different times after emergence, and how they were affected by male body mass and environmental condition (laboratory or semi-field arena). Sperm viability immediately after adult emergence showed no significant difference compared to four day old individuals, suggesting that O. cornuta males are capable of mating immediately post emergence. However, sperm counts were significantly higher in four day old individuals from the semi-field arena when compared to newly emerged males. This might reflect a final phase of sperm maturation. Regardless of individual male age and body mass differences, O. cornuta males produced on average ~175’000 spermatozoa that were ~65% viable, which are both significantly lower compared to eusocial honeybees and bumblebees. Moreover, sperm quantity, but not viability, was positively correlated with male body mass four days after emergence, while no such relationship was detected immediately after emergence. Even though individuals maintained in semi-field conditions exhibited a significantly greater loss of body mass, experimental arena had no significant effect on male survival, sperm quality or total living sperm produced. This suggests that the proposed laboratory design provides a cost-efficient and simple experimental approach to assess sperm traits in solitary bees. In conclusion, our data suggest a reduced investment in both sperm quantity and quality by male O. cornuta, which appears to be adaptive in light of the life history of this solitary bee.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Bee Health

UniBE Contributor:

Strobl, Verena; Straub, Lars; Kolari, Eleonora and Neumann, Peter

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Anna Papach

Date Deposited:

27 Mar 2020 08:49

Last Modified:

29 Mar 2020 03:43

Publisher DOI:

10.1371/journal.pone.0214597

PubMed ID:

30921425

BORIS DOI:

10.7892/boris.142085

URI:

https://boris.unibe.ch/id/eprint/142085

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