Liver-derived extracellular vesicles: A cell by cell overview to isolation and characterization practices

Zivko, Cristina; Fuhrmann, Gregor; Luciani, Paola (2020). Liver-derived extracellular vesicles: A cell by cell overview to isolation and characterization practices. Biochimica et biophysica acta (BBA) - general subjects, p. 129559. Elsevier 10.1016/j.bbagen.2020.129559

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Background Extracellular vesicles (EVs) are a diverse group of membrane-bound nanovesicles potentially released by every cell. With the liver's unique ensemble of cells and its fundamental physiological tasks, elucidating the role of EV-mediated hepatic cellular crosstalk and their role in different pathologies has been gaining the attention of many scientists. Scope of review The present review shifts the perspective into practice: we aim to critically discuss the methods used to purify and to biochemically analyse EVs from specific liver resident cells, including hepatocytes, hepatic stellate cells, cholangiocytes, liver sinusoidal endothelial cells, Kupffer cells, liver stem cells. The review offers a reference guide to current approaches. Major conclusions Strategies for EV isolation and characterization are as varied as the research groups performing them. We present main advantages and disadvantages for the methods, highlighting common causes for concern, such as FBS handling, reporting of cell viability, EV yield and storage, differences in differential centrifugations, suboptimal method descriptions, and method transferability. We both looked at how adaptable the research between human and rodent cells in vitro is, and also assessed how well either of them translates to ex vivo settings. General significance We reviewed methodological practices for the isolation and analysis of liver-derived EVs, making a cell type specific user guide that shows where to start, what has worked so far and to what extent. We critically discussed room for improvement, placing a particular focus on working towards a potential standardization of methods.

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Zivko, Cristina and Luciani, Paola

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics, prescription drugs

ISSN:

0304-4165

Publisher:

Elsevier

Funders:

[UNSPECIFIED] Phospholipid Research Center

Language:

English

Submitter:

Paola Luciani

Date Deposited:

25 Jun 2020 09:59

Last Modified:

30 Jun 2020 10:17

Publisher DOI:

10.1016/j.bbagen.2020.129559

BORIS DOI:

10.7892/boris.144838

URI:

https://boris.unibe.ch/id/eprint/144838

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