Associations of the intestinal microbiome with the complement system in neovascular age-related macular degeneration.

Zysset-Burri, Denise C.; Keller, Irene; Berger, Lieselotte E.; Largiadèr, Carlo R.; Wittwer, Matthias; Wolf, Sebastian; Zinkernagel, Martin S. (2020). Associations of the intestinal microbiome with the complement system in neovascular age-related macular degeneration. NPJ genomic medicine, 5(34), p. 34. Springer Nature 10.1038/s41525-020-00141-0

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Age-related macular degeneration (AMD) is a leading cause of severe vision loss in the aged population. The etiology of AMD is multifactorial including nutritional factors, genetic variants mainly in the complement pathway, environmental risk factors and alterations in the intestinal microbiome. However, it remains unexplored whether there is an interdependency of these factors leading to the development of AMD. To investigate this issue, a shotgun metagenomics analysis of 57 neovascular AMD and 58 healthy controls as well as of 16 complement C3-deficient mice and 16 wildtypes was performed. Whereas the class Negativicutes was more abundant in patients, the genus Oscillibacter and species Bacteroides had a significantly higher prevalence in persons without AMD. Similar taxonomic features were identified that distinguished wildtype mice from C3-deficient mice. Moreover, several purine signaling pathways were associated with both, neovascular AMD and C3 deficiency. While SNPs within the complement factor B gene were more abundant in controls, SNPs within the high temperature requirement A serine peptidase 1 and complement factor H (CFH) genes were associated with neovascular AMD. Using a classification model, Negativicutes was identified as a potential biomarker for AMD and furthermore, it positively correlated with CFH. This study suggests an association between the intestinal microbiome and the complement system in neovascular AMD.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde

UniBE Contributor:

Zysset-Burri, Denise Corinne; Keller, Irene; Berger, Lieselotte Erika; Largiadèr, Carlo Rodolfo; Wolf, Sebastian and Zinkernagel, Martin Sebastian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2056-7944

Publisher:

Springer Nature

Language:

English

Submitter:

Sebastian Wolf

Date Deposited:

07 Oct 2020 12:11

Last Modified:

11 Oct 2020 02:56

Publisher DOI:

10.1038/s41525-020-00141-0

PubMed ID:

32922859

Uncontrolled Keywords:

Macular degeneration Metagenomics

BORIS DOI:

10.7892/boris.146839

URI:

https://boris.unibe.ch/id/eprint/146839

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