Current problems and future avenues in proteoliposome research.

Amati, Andrea Marco; Graf, Simone; Deutschmann, Sabina; Dolder, Nicolas; von Ballmoos, Christoph (2020). Current problems and future avenues in proteoliposome research. Biochemical Society transactions, 48(4), pp. 1473-1492. Portland Press 10.1042/BST20190966

[img]
Preview
Text
Amati et al_accepted_manuscript.pdf - Accepted Version
Available under License Publisher holds Copyright.

Download (2MB) | Preview

Membrane proteins (MPs) are the gatekeepers between different biological compartments separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they fulfill a wide variety of cellular functions and their importance is reflected in the increasing number of drugs that target MPs. Functional studies of MPs within a native cellular context, however, is difficult due to the innate complexity of the densely packed membranes. Over the past decades, detergent-based extraction and purification of MPs and their reconstitution into lipid mimetic systems has been a very powerful tool to simplify the experimental system. In this review, we focus on proteoliposomes that have become an indispensable experimental system for enzymes with a vectorial function, including many of the here described energy transducing MPs. We first address long standing questions on the difficulty of successful reconstitution and controlled orientation of MPs into liposomes. A special emphasis is given on coreconstitution of several MPs into the same bilayer. Second, we discuss recent progress in the development of fluorescent dyes that offer sensitive detection with high temporal resolution. Finally, we briefly cover the use of giant unilamellar vesicles for the investigation of complex enzymatic cascades, a very promising experimental tool considering our increasing knowledge of the interplay of different cellular components.

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Amati, Andrea Marco; Graf, Simone Sandra; Deutschmann, Sabina; Dolder, Nicolas and von Ballmoos, Christoph

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0300-5127

Publisher:

Portland Press

Language:

English

Submitter:

Christoph von Ballmoos

Date Deposited:

03 Nov 2020 09:32

Last Modified:

25 Aug 2021 02:30

Publisher DOI:

10.1042/BST20190966

PubMed ID:

32830854

Uncontrolled Keywords:

liposome membrane mimicking system membrane proteins protein reconstitution synthetic biology

BORIS DOI:

10.7892/boris.147124

URI:

https://boris.unibe.ch/id/eprint/147124

Actions (login required)

Edit item Edit item
Provide Feedback