Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy

Dwapanyin, George; Spangenberg, Dirk; Heidt, Alexander; Feurer, Thomas; Bosman, Gurthwin; Neethling, Pieter; Rohwer, Erich (2020). Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy. Journal of the Optical Society of America. B - optical physics, 37(11), A285-A292. Optical Society of America 10.1364/JOSAB.396938

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Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, i2PIE, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, i2PIE leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Institute of Applied Physics
08 Faculty of Science > Institute of Applied Physics > Lasers

UniBE Contributor:

Spangenberg, Dirk-Mathys; Heidt, Alexander and Feurer, Thomas

Subjects:

500 Science > 530 Physics
600 Technology > 620 Engineering

ISSN:

0740-3224

Publisher:

Optical Society of America

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Alexander Heidt

Date Deposited:

05 Nov 2020 14:43

Last Modified:

05 Nov 2020 14:43

Publisher DOI:

10.1364/JOSAB.396938

BORIS DOI:

10.7892/boris.147272

URI:

https://boris.unibe.ch/id/eprint/147272

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