Chlamydia-induced curvature of the host-cell plasma membrane is required for infection.

Hänsch, Sebastian; Spona, Dominik; Murra, Gido; Köhrer, Karl; Subtil, Agathe; Furtado, Ana Rita; Lichtenthaler, Stephan F; Dislich, Bastian; Mölleken, Katja; Hegemann, Johannes H (2020). Chlamydia-induced curvature of the host-cell plasma membrane is required for infection. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 117(5), pp. 2634-2644. National Academy of Sciences NAS 10.1073/pnas.1911528117

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During invasion of host cells, Chlamydia pneumoniae secretes the effector protein CPn0678, which facilitates internalization of the pathogen by remodeling the target cell's plasma membrane and recruiting sorting nexin 9 (SNX9), a central multifunctional endocytic scaffold protein. We show here that the strongly amphipathic N-terminal helix of CPn0678 mediates binding to phospholipids in both the plasma membrane and synthetic membranes, and is sufficient to induce extensive membrane tubulations. CPn0678 interacts via its conserved C-terminal polyproline sequence with the Src homology 3 domain of SNX9. Thus, SNX9 is found at bacterial entry sites, where C. pneumoniae is internalized via EGFR-mediated endocytosis. Moreover, depletion of human SNX9 significantly reduces internalization, whereas ectopic overexpression of CPn0678-GFP results in a dominant-negative effect on endocytotic processes in general, leading to the uptake of fewer chlamydial elementary bodies and diminished turnover of EGFR. Thus, CPn0678 is an early effector involved in regulating the endocytosis of C. pneumoniae in an EGFR- and SNX9-dependent manner.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Dislich, Bastian

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0027-8424

Publisher:

National Academy of Sciences NAS

Language:

English

Submitter:

Bastian Dislich

Date Deposited:

12 Nov 2020 11:40

Last Modified:

12 Nov 2020 11:40

Publisher DOI:

10.1073/pnas.1911528117

PubMed ID:

31964834

Uncontrolled Keywords:

effector protein endocytosis lipid binding membrane modulation

BORIS DOI:

10.7892/boris.147837

URI:

https://boris.unibe.ch/id/eprint/147837

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