A novel bioreactor system capable of simulating the in vivo conditions of synovial joints.

Tekari, Adel; Egli, Rainer J.; Schmid, Veit; Justiz, Joern; Luginbuehl, Reto (2020). A novel bioreactor system capable of simulating the in vivo conditions of synovial joints. Tissue engineering. Part C, Methods, 26(12), pp. 617-627. Mary Ann Liebert 10.1089/ten.TEC.2020.0161

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Any significant in vitro evaluation of cartilage tissue engineering and cartilage repair strategies has to be performed under the harsh conditions encountered in vivo within synovial joints. To this end, we have developed a novel automated physiological robot reactor system (PRRS) capable of recapitulating complex physiological motions and load patterns within an environment similar to that found in the human knee. The PRRS consists of a mechanical stimulation unit (MSU) and an automatic sample changer (ASC) within an environment control box (ECB) in which the humidity, temperature, and gas composition are tightly regulated. The MSU has three linear (orthogonal) axes and one rotational degree of freedom (around the z-axis). The ASC provides space for up to 24 samples, which can be allocated to individual stimulation patterns. Cell-seeded scaffolds and ex vivo tissue culture systems were established to demonstrate the applicability of the PRRS to the investigation of the effect of load and environmental conditions on engineering and maintenance of articular cartilage in vitro. The bioreactor is a flexible system that has the potential to be applied for culturing connective tissues other than cartilage, such as bone and intervertebral disc tissue, even though the mechanical and environmental parameters are very different.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Tekari, Adel; Egli, Rainer Josef and Luginbühl, Reto

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1937-3392

Publisher:

Mary Ann Liebert

Language:

English

Submitter:

Maria de Fatima Henriques Bernardo

Date Deposited:

29 Dec 2020 17:09

Last Modified:

29 Dec 2020 17:18

Publisher DOI:

10.1089/ten.TEC.2020.0161

PubMed ID:

33267725

BORIS DOI:

10.48350/149063

URI:

https://boris.unibe.ch/id/eprint/149063

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