SDF-1/CXCR4 signalling is involved in blood vessel growth and remodelling by intussusception.

Dimova, Ivanka; Karthik, Swapna; Makanya, Andrew; Hlushchuk, Ruslan; Semela, David; Volarevic, Vladislav; Djonov, Valentin (2019). SDF-1/CXCR4 signalling is involved in blood vessel growth and remodelling by intussusception. Journal of Cellular and Molecular Medicine, 23(6), pp. 3916-3926. Wiley 10.1111/jcmm.14269

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The precise mechanisms of SDF-1 (CXCL12) in angiogenesis are not fully elucidated. Recently, we showed that Notch inhibition induces extensive intussusceptive angiogenesis by recruitment of mononuclear cells and it was associated with increased levels of SDF-1 and CXCR4. In the current study, we demonstrated SDF-1 expression in liver sinusoidal vessels of Notch1 knockout mice with regenerative hyperplasia by means of intussusception, but we did not detect any SDF-1 expression in wild-type mice with normal liver vessel structure. In addition, pharmacological inhibition of SDF-1/CXCR4 signalling by AMD3100 perturbs intussusceptive vascular growth and abolishes mononuclear cell recruitment in the chicken area vasculosa. In contrast, treatment with recombinant SDF-1 protein increased microvascular density by 34% through augmentation of pillar number compared to controls. The number of extravasating mononuclear cells was four times higher after SDF-1 application and two times less after blocking this pathway. Bone marrow-derived mononuclear cells (BMDC) were recruited to vessels in response to elevated expression of SDF-1 in endothelial cells. They participated in formation and stabilization of pillars. The current study is the first report to implicate SDF-1/CXCR4 signalling in intussusceptive angiogenesis and further highlights the stabilizing role of BMDC in the formation of pillars during vascular remodelling.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Karthik, Swapna; Makanya, Andrew; Hlushchuk, Ruslan; Volarevic, Vladislav and Djonov, Valentin Georgiev

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1582-1838

Publisher:

Wiley

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

David Christian Haberthür

Date Deposited:

30 Dec 2020 09:37

Last Modified:

31 Dec 2020 13:22

Publisher DOI:

10.1111/jcmm.14269

PubMed ID:

30950188

Uncontrolled Keywords:

SDF-1/CXCR4 signalling bone marrow-derived mononuclear cells intussusceptive angiogenesis vessel remodelling

BORIS DOI:

10.48350/149217

URI:

https://boris.unibe.ch/id/eprint/149217

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