Contat, Caroline; Ancey, Pierre-Benoit; Zangger, Nadine; Sabatino, Silvia; Pascual, Justine; Escrig, Stéphane; Jensen, Louise; Goepfert, Christine; Lanz, Bernard; Lepore, Mario; Gruetter, Rolf; Rossier, Anouk; Berezowska, Sabina; Neppl, Christina; Zlobec, Inti; Clerc-Rosset, Stéphanie; Knott, Graham William; Rathmell, Jeffrey C; Abel, E Dale; Meibom, Anders; ... (2020). Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth. eLife, 9 eLife Sciences Publications 10.7554/eLife.53618
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Glucose utilization increases in tumors, a metabolic process that is observed clinically by 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET). However, is increased glucose uptake important for tumor cells, and which transporters are implicated in vivo? In a genetically-engineered mouse model of lung adenocarcinoma, we show that the deletion of only one highly expressed glucose transporter, Glut1 or Glut3, in cancer cells does not impair tumor growth, whereas their combined loss diminishes tumor development. 18F-FDG-PET analyses of tumors demonstrate that Glut1 and Glut3 loss decreases glucose uptake, which is mainly dependent on Glut1. Using 13C-glucose tracing with correlated nanoscale secondary ion mass spectrometry (NanoSIMS) and electron microscopy, we also report the presence of lamellar body-like organelles in tumor cells accumulating glucose-derived biomass, depending partially on Glut1. Our results demonstrate the requirement for two glucose transporters in lung adenocarcinoma, the dual blockade of which could reach therapeutic responses not achieved by individual targeting.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Pathology > Translational Research Unit 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology |
UniBE Contributor: |
Göpfert, Christine, Zlobec, Inti |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2050-084X |
Publisher: |
eLife Sciences Publications |
Language: |
English |
Submitter: |
Inti Zlobec |
Date Deposited: |
11 Mar 2021 12:20 |
Last Modified: |
05 Dec 2022 15:43 |
Publisher DOI: |
10.7554/eLife.53618 |
PubMed ID: |
32571479 |
Uncontrolled Keywords: |
NanoSIMS cancer biology genetically engineered mouse model of cancer glucose transporters human lamellar bodies lung adenocarcinoma mouse |
BORIS DOI: |
10.48350/150077 |
URI: |
https://boris.unibe.ch/id/eprint/150077 |
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