Computer simulation of the isotachophoretic migration and separation of norpseudoephedrine stereoisomers with a free or immobilized neutral chiral selector.

Caslavsky, Jitka; Mosher, Richard A; Thormann, Wolfgang (2020). Computer simulation of the isotachophoretic migration and separation of norpseudoephedrine stereoisomers with a free or immobilized neutral chiral selector. Journal of chromatography A, 1623, p. 461176. Elsevier Science 10.1016/j.chroma.2020.461176

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A detailed computer simulation study of the isotachophoretic migration and separation of norpseudoephedrine stereoisomers for cases with the neutral selector added to the leader, immobilized to the capillary wall or support, or partially present in the separation column is presented. The electrophoretic transport of the analytes from the sampling compartment into the separation medium with the selector, the formation of a transient mixed zone, the separation dynamics of the stereoisomers with a free or immobilized selector, the dependence of the leader pH, the ionic mobility of norpseudoephedrine, the complexation constant and selector immobilization on steady-state plateau zone properties, and zone changes occurring during the transition from the chiral environment into a selector free leader are thereby visualized in a hitherto unexplored way. For the case with the selector dissolved in the leading electrolyte, simulation data are compared to those observed in experimental setups with coated fused-silica capillaries that feature minimized electroosmosis and zone detection with conductivity and absorbance detectors.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Caslavsky, Jitka and Thormann, Wolfgang

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0021-9673

Publisher:

Elsevier Science

Language:

English

Submitter:

Wolfgang Thormann

Date Deposited:

19 Jan 2021 15:38

Last Modified:

17 Mar 2021 03:25

Publisher DOI:

10.1016/j.chroma.2020.461176

PubMed ID:

32505280

Uncontrolled Keywords:

Chiral separation Computer simulation Cyclodextrin Electrokinetic chromatography Electrophoresis Isotachophoresis

BORIS DOI:

10.48350/150383

URI:

https://boris.unibe.ch/id/eprint/150383

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