Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys.

Unger, Lucia; Abril, Carlos; Gerber, Vinzenz; Jagannathan, Vidhya; Koch, Christoph; Hamza, Eman (2021). Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys. Journal of veterinary internal medicine, 35(1), pp. 610-619. Wiley-Blackwell 10.1111/jvim.16027

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BACKGROUND

MicroRNAs (miRNAs) are potential biomarkers for equine sarcoids (ES).

OBJECTIVES

To assess eca-miR-331, eca-miR-100, and eca-miR-1 as serum biomarkers for ES disease.

ANIMALS

Sixty-eight ES cases (56 horses, 12 donkeys), 69 tumor-free controls (60 horses, 9 donkeys), and 20 horses with other skin tumors.

METHODS

For this case-control study, expression of serum eca-miR-331, eca-miR-100, and eca-miR-1 in ES-affected equids was compared to tumor-free age-, sex-, and breed-matched control horses and donkeys with other skin tumors using reverse transcription quantitative PCR (polymerase chain reaction) for relative miRNA quantification. Biological, preanalytical, and clinical variable influences on miRNA expression were examined. Receiver operator characteristic (ROC) curve analyses were used to determine differences in miRNA expression between groups.

RESULTS

The expression of eca-miR-100 was affected by age (P = .003) and expression of eca-miR-100 and eca-miR-1 were affected by hemolysis (both P < .001). Eca-miR-331 was unaffected by biological variation, hemolysis, ES type, and disease severity. Eca-miR-331 concentrations were higher in ES-affected compared to tumor-free controls (P = .002). The ROC curve analysis indicated an area under the curve of 0.65 (P = .002) with a sensitivity of 60%, specificity of 71%, and positive and negative likelihood ratios of 2.1 and 0.56, respectively, to diagnose ES. Eca-miR-331 expression did not discriminate between horses with ES and other skin tumors. Expression of eca-miR-100 and eca-miR-1 was not different between groups.

CONCLUSIONS AND CLINICAL IMPORTANCE

Serum eca-miR-331 expression is neither sensitive nor specific enough as a single ES biomarker. If combined with other miRNAs, it may be helpful for ES diagnosis.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Unger, Lucia; Gerber, Vinzenz; Jagannathan, Vidya; Koch, Christoph and Hamza, Eman

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0891-6640

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Ursula Therese Horst

Date Deposited:

18 Jan 2021 09:25

Last Modified:

12 Mar 2021 10:09

Publisher DOI:

10.1111/jvim.16027

PubMed ID:

33415768

Uncontrolled Keywords:

circulating microRNA eca-miR-331 equine tumor marker transcriptomic biomarker

BORIS DOI:

10.48350/151361

URI:

https://boris.unibe.ch/id/eprint/151361

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