An aminoacylation independent activity of PheRS/FARS promotes growth and proliferation

Ho, Manh Tin; Lu, Jiongming; Brunssen, Dominique Yvonne; Suter, Beat (2021). An aminoacylation independent activity of PheRS/FARS promotes growth and proliferation. Disease models & mechanisms, 14(3), dmm048132. Company of Biologists Ltd. 10.1242/dmm.048132

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Aminoacyl tRNA synthetases (aaRSs) not only load the appropriate amino acid onto their cognate tRNA, but many of them also perform additional functions that are not necessarily related to their canonical activities. Phenylalanyl tRNA synthetase (PheRS/FARS) levels are elevated in multiple cancers compared to their normal cell counterparts. Our results now show that downregulation of PheRS, or only its -PheRS subunit, reduces organ size, whereas elevated expression of the -PheRS subunit stimulates cell growth and proliferation. In the wing discs system, this can lead to a 67% increase of cells that stain for a mitotic marker. Clonal analysis of twin spots in the follicle cells of the ovary revealed that the elevated expression of the -PheRS subunit causes cells to grow and proliferate about 25% faster than their normal twin cells. And this faster growth and proliferation did not affect the size distribution of the proliferating cells. Importantly, this stimulation proliferation turned out to be independent of the β-PheRS subunit and the aminoacylation activity, and it did not visibly stimulate translation.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Drosophila
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ho, Manh Tin; Lu, Jiongming; Brunssen, Dominique Yvonne and Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1754-8403

Publisher:

Company of Biologists Ltd.

Funders:

[42] Schweizerischer Nationalfonds ; [UNSPECIFIED] Novartis Stiftung für Medizinisch- Biologische Forschung ; [UNSPECIFIED] Universität Bern

Language:

English

Submitter:

Beat Suter

Date Deposited:

25 Jan 2021 11:42

Last Modified:

21 Mar 2021 02:59

Publisher DOI:

10.1242/dmm.048132

URI:

https://boris.unibe.ch/id/eprint/151519

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