Phylogenomics of Mycobacterium africanum reveals a new lineage and a complex evolutionary history.

Coscolla, Mireia; Gagneux, Sebastien; Menardo, Fabrizio; Loiseau, Chloé; Ruiz-Rodriguez, Paula; Borrell, Sonia; Otchere, Isaac Darko; Asante-Poku, Adwoa; Asare, Prince; Sánchez-Busó, Leonor; Gehre, Florian; Sanoussi, C N'Dira; Antonio, Martin; Affolabi, Dissou; Fyfe, Janet; Beckert, Patrick; Niemann, Stefan; Alabi, Abraham S; Grobusch, Martin P; Kobbe, Robin; ... (2021). Phylogenomics of Mycobacterium africanum reveals a new lineage and a complex evolutionary history. Microbial genomics, 7(2) Microbiology Society 10.1099/mgen.0.000477

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Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC). The MTBC comprises several human-adapted lineages known as M. tuberculosis sensu stricto, as well as two lineages (L5 and L6) traditionally referred to as Mycobacterium africanum. Strains of L5 and L6 are largely limited to West Africa for reasons unknown, and little is known of their genomic diversity, phylogeography and evolution. Here, we analysed the genomes of 350 L5 and 320 L6 strains, isolated from patients from 21 African countries, plus 5 related genomes that had not been classified into any of the known MTBC lineages. Our population genomic and phylogeographical analyses showed that the unclassified genomes belonged to a new group that we propose to name MTBC lineage 9 (L9). While the most likely ancestral distribution of L9 was predicted to be East Africa, the most likely ancestral distribution for both L5 and L6 was the Eastern part of West Africa. Moreover, we found important differences between L5 and L6 strains with respect to their phylogeographical substructure and genetic diversity. Finally, we could not confirm the previous association of drug-resistance markers with lineage and sublineages. Instead, our results indicate that the association of drug resistance with lineage is most likely driven by sample bias or geography. In conclusion, our study sheds new light onto the genomic diversity and evolutionary history of M. africanum, and highlights the need to consider the particularities of each MTBC lineage for understanding the ecology and epidemiology of TB in Africa and globally.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine (ISPM)

UniBE Contributor:

Fenner, Lukas

Subjects:

600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services

ISSN:

2057-5858

Publisher:

Microbiology Society

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Doris Kopp Heim

Date Deposited:

09 Feb 2021 16:11

Last Modified:

13 Mar 2021 20:41

Publisher DOI:

10.1099/mgen.0.000477

PubMed ID:

33555243

Uncontrolled Keywords:

Mycobacterium africanum Mycobacterium tuberculosis diversity evolution genome mycobacteria

BORIS DOI:

10.48350/152081

URI:

https://boris.unibe.ch/id/eprint/152081

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