Novotny, Ella N.; White, Samuel J.; Wilson, A. Douglas; Stefánsdóttir, Sara B.; Tijhaar, Edwin; Jonsdóttir, Sigridur; Frey, Rebekka; Reiche, Dania; Rose, Horst; Rhyner, Claudio; Schüpbach, Gertraud; Torsteinsdóttir, Sigurbjörg; Alcocer, Marcos; Marti, Eliane (2021). Component‐resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity. Allergy, 76(4), pp. 1147-1157. Wiley-Blackwell 10.1111/all.14556
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Background: Allergy to bites of blood-sucking insects, including biting midges, can affect both human and veterinary patients. Horses are often suffering from an IgE-mediated allergic dermatitis caused by bites of midges (Culicoides spp). With the aim to improve allergen immunotherapy (AIT), numerous Culicoides allergens have been produced as recombinant (r-) proteins. This study aimed to test a comprehensive panel of differently expressed Culicoides r-allergens on a cohort of IBH-affected and control horses using an allergen microarray.
Methods: IgE levels to 27 Culicoides r-allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut-offs selected using a specificity of 95% and seropositivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r-allergens giving the best performing test was determined using logistic regression analysis.
Results: Seropositivity was significantly higher in IBH horses compared with controls for 25 r-allergens. Nine Culicoides r-allergens were major allergens for IBH with seven of them binding IgE in sera from > 70% of the IBH-affected horses. Combination of these top seven r-allergens could diagnose > 90% of IBH-affected horses with a specificity of > 95%. Correlation between differently expressed r-allergens was usually high (mean = 0.69, range: 0.28-0.91).
Conclusion: This microarray will be a powerful tool for the development of component-resolved, patient-tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Novotny, Ella Nina, Jonsdottir, Sigridur, Schüpbach-Regula, Gertraud Irene, Marti, Eliane Isabelle |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture 500 Science > 590 Animals (Zoology) |
ISSN: |
0105-4538 |
Publisher: |
Wiley-Blackwell |
Language: |
English |
Submitter: |
Eliane Isabelle Marti Schalch |
Date Deposited: |
23 Mar 2021 15:40 |
Last Modified: |
02 Mar 2023 23:34 |
Publisher DOI: |
10.1111/all.14556 |
PubMed ID: |
32780483 |
BORIS DOI: |
10.48350/153057 |
URI: |
https://boris.unibe.ch/id/eprint/153057 |
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