Maksimov, Pavlo; Bergmann, Hannes; Wassermann, Marion; Romig, Thomas; Gottstein, Bruno; Casulli, Adriano; Conraths, Franz J (2020). Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs. Pathogens, 9(10) MDPI AG 10.3390/pathogens9100791
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Infections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the E. granulosus s.l. complex. It is therefore essential to diagnose the correct species within E. granulosus s.l. to help understand specific disease epidemiology and to facilitate effective implementation of control measures. For this purpose, simple, fast and cost-effective typing techniques are needed. We developed quantitative real-time polymerase chain reactions (qPCRs) to target polymorphic regions in the mitochondrial genome of E. granulosus s.l. In a single-step typing approach, we distinguished E. granulosus s.l. members in four epidemiologically relevant subgroups. These were E. granulosus sensu stricto, E. equinus, E. ortleppi and the E. canadensis cluster. The technique also allowed identification and differentiation of these species from other Echinococcus or Taenia taxa for samples isolated from cysts or faeces.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Gottstein, Bruno |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture |
ISSN: |
2076-0817 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Pamela Schumacher |
Date Deposited: |
17 Mar 2021 09:57 |
Last Modified: |
05 Dec 2022 15:49 |
Publisher DOI: |
10.3390/pathogens9100791 |
PubMed ID: |
32993077 |
Uncontrolled Keywords: |
DNA probe Echinococcus granulosus sensu lato species diagnosis real-time polymerase chain reaction |
BORIS DOI: |
10.48350/154118 |
URI: |
https://boris.unibe.ch/id/eprint/154118 |
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