Does Aldosterone participate in placental angiogenesis via PLGF?

Eisele, Nicole; Gennari, Carine; Albrecht, Christiane; Baumann, Marc; Surbek, Daniel; Mohaupt, Markus (2012). Does Aldosterone participate in placental angiogenesis via PLGF? Pregnancy hypertension, 2(3), p. 245. Amsterdam: Elsevier Science 10.1016/j.preghy.2012.04.121

[img] Text
1-s2.0-S221077891200150X-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (36kB)

Introduction Angiogenic signals are a vital signal of placental integrity. Aldosterone has recently been shown to enhance placental growth factor (PlGF) expression in the peripheral vasculature [1] and to promote trophoblast growth [2]. The plgf gene possesses a functional mineralocorticoid receptor responsive element in the promoter region. Objectives Thus, we hypothesized that aldosterone adapts placental angiogenesis to trophoblast growth by secreting PlGF. Methods The human choriocarcinoma cell line BeWo and first and third trimester human primary trophoblasts cells were subjected to several syncytialization signals. Upon visual confirmation, the cultured cells were subjected to either control conditions, the known stimulator forskolin, and increasing amounts of aldosterone (10−9 to 10−6 M) with and without the competitive aldosterone receptor blocker spironolactone. After 6 and 24 h of incubation, RNA and protein were extracted. PlGF transcripts were quantified by Taqman PCR normalized to several housekeeping genes. Protein expression was quantified by ELISA. Results PlGF mRNA expression increased 3-fold with forskolin in BeWo cells. In this cell line, aldosterone could slightly stimulate PlGF production. In non-syncytialized primary human first trimester trophoblasts, aldosterone did not exert a specific effect. In contrast, the term primary human trophoblasts did respond with a 2.5-fold increase after incubation with aldosterone (10−7 M) in the presence of forskolin to allow forming a syncytial layer. PlGF protein was already slightly upregulated following 6 h of incubation with aldosterone. Conclusion We concluded that aldosterone does regulate PlGF expression in specified conditions during pregnancy. Inappropriately low aldosterone levels such as in preeclampsia might such not only compromise plasma volume and trophoblast growth but also placental vascularization and systemic PlGF availability. These observations merit further investigation.

Item Type:

Conference or Workshop Item (Abstract)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

UniBE Contributor:

Eisele, Nicole; Gennari, Carine; Albrecht, Christiane; Baumann, Marc; Surbek, Daniel and Mohaupt, Markus

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2210-7789

Publisher:

Elsevier Science

Language:

English

Submitter:

Markus Georg Mohaupt

Date Deposited:

04 Oct 2013 14:38

Last Modified:

08 May 2016 10:06

Publisher DOI:

10.1016/j.preghy.2012.04.121

BORIS DOI:

10.7892/boris.15526

URI:

https://boris.unibe.ch/id/eprint/15526 (FactScience: 222890)

Actions (login required)

Edit item Edit item
Provide Feedback