Herpesvirus-Associated Proliferative Skin Disease in Frogs and Toads: Proposed Pathogenesis.

Origgi, Francesco C.; Otten, Patricia; Lohmann, Petra; Sattler, Ursula; Wahli, Thomas; Lavazza, Antonio; Gaschen, Véronique; Stoffel, Michael H. (2021). Herpesvirus-Associated Proliferative Skin Disease in Frogs and Toads: Proposed Pathogenesis. (In Press). Veterinary pathology, p. 3009858211006385. Sage 10.1177/03009858211006385

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A comparative study was carried out on common and agile frogs (Rana temporaria and R. dalmatina) naturally infected with ranid herpesvirus 3 (RaHV3) and common toads (Bufo bufo) naturally infected with bufonid herpesvirus 1 (BfHV1) to investigate common pathogenetic pathways and molecular mechanisms based on macroscopic, microscopic, and ultrastructural pathology as well as evaluation of gene expression. Careful examination of the tissue changes, supported by in situ hybridization, at different stages of development in 6 frogs and 14 toads revealed that the skin lesions are likely transient, and part of a tissue cycle necessary for viral replication in the infected hosts. Transcriptomic analysis, carried out on 2 naturally infected and 2 naïve common frogs (Rana temporaria) and 2 naturally infected and 2 naïve common toads (Bufo bufo), revealed altered expression of genes involved in signaling and cell remodeling in diseased animals. Finally, virus transcriptomics revealed that both RaHV3 and BfHV1 had relatively high expression of a putative immunomodulating gene predicted to encode a decoy receptor for tumor necrosis factor in the skin of the infected hosts. Thus, the comparable lesions in infected frogs and toads appear to reflect a concerted epidermal and viral cycle, with presumptive involvement of signaling and gene remodeling host and immunomodulatory viral genes.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Origgi, Francesco; Sattler, Ursula; Wahli, Thomas; Gaschen, Véronique and Stoffel, Michael Hubert

Subjects:

500 Science
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

1544-2217

Publisher:

Sage

Language:

English

Submitter:

Vanessa Alice Blum

Date Deposited:

19 Apr 2021 14:32

Last Modified:

20 Apr 2021 01:35

Publisher DOI:

10.1177/03009858211006385

PubMed ID:

33813961

Uncontrolled Keywords:

amphibians, emerging diseases, herpesvirus, pathogenesis, pathology, wildlife

BORIS DOI:

10.48350/155932

URI:

https://boris.unibe.ch/id/eprint/155932

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