Hijacking of the host cell Golgi by Plasmodium berghei liver stage parasites

De Niz, Mariana; Caldelari, Reto; Kaiser, Gesine; Zuber, Benoît; Heo, Won Do; Heussler, Volker T.; Agop-Nersesian, Carolina (2021). Hijacking of the host cell Golgi by Plasmodium berghei liver stage parasites. Journal of cell science, 134(10) Company of Biologists Limited 10.1242/jcs.252213

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he intracellular lifestyle represents a challenge for the rapidly proliferating liver stage Plasmodium parasite. In order to scavenge host resources, Plasmodium has evolved the ability to target and manipulate host cell organelles. Using dynamic fluorescence-based imaging, we here show an interplay between the pre-erythrocytic stages of Plasmodium berghei and the host cell Golgi during liver stage development. Liver stage schizonts fragment the host cell Golgi into miniaturized stacks, which increases surface interactions with the parasitophorous vacuolar membrane of the parasite. Expression of specific dominant-negative Arf1 and Rab GTPases, which interfere with the host cell Golgi-linked vesicular machinery, results in developmental delay and diminished survival of liver stage parasites. Moreover, functional Rab11a is critical for the ability of the parasites to induce Golgi fragmentation. Altogether, we demonstrate that the structural integrity of the host cell Golgi and Golgi-associated vesicular traffic is important for optimal pre-erythrocytic development of P. berghei. The parasite hijacks the Golgi structure of the hepatocyte to optimize its own intracellular development.

Item Type:

Journal Article (Original Article)

Division/Institute:

09 Interdisciplinary Units > Microscopy Imaging Center (MIC)
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

De Niz Hidalgo, Mariana Isabel; Caldelari, Reto; Kaiser, Gesine; Zuber, Benoît; Heussler, Volker and Agop Nersesian, Carolina

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0021-9533

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

31 May 2021 10:26

Last Modified:

31 May 2021 10:26

Publisher DOI:

10.1242/jcs.252213

PubMed ID:

34013963

BORIS DOI:

10.48350/156245

URI:

https://boris.unibe.ch/id/eprint/156245

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