Biocatalytic access to betazole by one-pot multienzymatic system in continuous flow

Romero-Fernandez, Maria; Paradisi, Francesca (2021). Biocatalytic access to betazole by one-pot multienzymatic system in continuous flow. Green chemistry, 23(12), pp. 4594-4603. Royal Society of Chemistry 10.1039/D1GC01095F

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As an alternative to classical synthetic approaches for the production of betazole drug, a one-pot biocatalytic system to this pharmaceutical molecule from its alcohol precursor has been developed. An ꙍ-transaminase, an alcohol dehydrogenase and a water-forming NADH oxidase for in-situ cofactor recycling have been combined to catalyse this reaction yielding 75% molar conversion in batch reaction with soluble enzymes. This multienzyme system was then co-immobilised through a newly established protocol for sequential functionalization of a methacrylate-based porous carrier to enable tailored immobilisation chemistries for each enzyme. This pluri-catalytic system has been set up in a continuous flow packed-bed reactor, yielding a space-time yield up to 2.59 g L-1 h-1 with 15 min residence with constant supply of oxygen for in-situ cofactor recycling through a segmented air-liquid flow. The addition of an in-line catch-and-release column afforded >80% product recovery.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Paradisi, Francesca

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1463-9262

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Francesca Paradisi

Date Deposited:

03 Jun 2021 15:10

Last Modified:

22 Jun 2021 01:34

Publisher DOI:

10.1039/D1GC01095F

BORIS DOI:

10.48350/156586

URI:

https://boris.unibe.ch/id/eprint/156586

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