Romero-Fernandez, Maria; Paradisi, Francesca (2021). Biocatalytic access to betazole by one-pot multienzymatic system in continuous flow. Green chemistry, 23(12), pp. 4594-4603. Royal Society of Chemistry 10.1039/D1GC01095F
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As an alternative to classical synthetic approaches for the production of betazole drug, a one-pot biocatalytic system to this pharmaceutical molecule from its alcohol precursor has been developed. An ꙍ-transaminase, an alcohol dehydrogenase and a water-forming NADH oxidase for in-situ cofactor recycling have been combined to catalyse this reaction yielding 75% molar conversion in batch reaction with soluble enzymes. This multienzyme system was then co-immobilised through a newly established protocol for sequential functionalization of a methacrylate-based porous carrier to enable tailored immobilisation chemistries for each enzyme. This pluri-catalytic system has been set up in a continuous flow packed-bed reactor, yielding a space-time yield up to 2.59 g L-1 h-1 with 15 min residence with constant supply of oxygen for in-situ cofactor recycling through a segmented air-liquid flow. The addition of an in-line catch-and-release column afforded >80% product recovery.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Paradisi, Francesca |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1463-9262 |
Publisher: |
Royal Society of Chemistry |
Language: |
English |
Submitter: |
Francesca Paradisi |
Date Deposited: |
03 Jun 2021 15:10 |
Last Modified: |
05 Dec 2022 15:51 |
Publisher DOI: |
10.1039/D1GC01095F |
BORIS DOI: |
10.48350/156586 |
URI: |
https://boris.unibe.ch/id/eprint/156586 |
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