Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes.

Welner, Simon; Ruggli, Nicolas; Liniger, Matthias; Summerfield, Artur; Larsen, Lars Erik; Jungersen, Gregers (2021). Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes. Vaccines, 9(3) MDPI 10.3390/vaccines9030208

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Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe respiratory distress and reproductive failure in swine. Modified live virus (MLV) vaccines provide the highest degree of protection and are most often the preferred choice. While somewhat protective, the use of MLVs is accompanied by multiple safety issues, why safer alternatives are urgently needed. Here, we describe the generation of virus replicon particles (VRPs) based on a classical swine fever virus genome incapable of producing infectious progeny and designed to express conserved PRRSV-2 cytotoxic T-cell epitopes. Eighteen pigs matched with the epitopes by their swine leucocyte antigen-profiles were vaccinated (N = 11, test group) or sham-vaccinated (N = 7, control group) with the VRPs and subsequently challenged with PRRSV-2. The responses to vaccination and challenge were monitored using serological, immunological, and virological analyses. Challenge virus load in serum did not differ significantly between the groups, whereas the virus load in the caudal part of the lung was significantly lower in the test group compared to the control group. The number of peptide-induced interferon-γ secreting cells after challenge was higher and more frequent in the test group than in the control group. Together, our results provide indications of a shapeable PRRSV-specific cell-mediated immune response that may inspire future development of effective PRRSV vaccines.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Ruggli, Nicolas; Liniger, Matthias and Summerfield, Artur

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

2076-393X

Publisher:

MDPI

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

13 Jul 2021 09:41

Last Modified:

13 Jul 2021 09:41

Publisher DOI:

10.3390/vaccines9030208

PubMed ID:

33801369

Uncontrolled Keywords:

cell-mediated immunity classical swine fever virus (CSFV) cytotoxic T cells polyepitope antigen porcine reproductive and respiratory syndrome virus (PRRSV) vaccine viral vector virus replicon particles (VRP)

BORIS DOI:

10.48350/156604

URI:

https://boris.unibe.ch/id/eprint/156604

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