The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin.

Guensch, Dominik P.; Michel, Matthias C.; Huettenmoser, Stefan P; Jung, Bernd; Gulac, Patrik; Segiser, Adrian; Longnus, Sarah L.; Fischer, Kady (2021). The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin. Scientific reports, 11(1), p. 11464. Springer Nature 10.1038/s41598-021-90908-x

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The presence of deoxygenated hemoglobin (Hb) results in a drop in T2 and T2* in magnetic resonance imaging (MRI), known as the blood oxygenation level-dependent (BOLD-)effect. The purpose of this study was to investigate if deoxygenated myoglobin (Mb) exerts a BOLD-like effect. Equine Met-Mb powder was dissolved and converted to oxygenated Mb. T1, T2, T2*-maps and BOLD-bSSFP images at 3Tesla were used to scan 22 Mb samples and 12 Hb samples at room air, deoxygenation, reoxygenation and after chemical reduction. In Mb, T2 and T2* mapping showed a significant decrease after deoxygenation (- 25% and - 12%, p < 0.01), increase after subsequent reoxygenation (+ 17% and 0% vs. room air, p < 0.01), and finally a decrease in T2 after chemical reduction (- 28%, p < 0.01). An opposite trend was observed with T1 for each stage, while chemical reduction reduced BOLD-bSSFP signal (- 3%, p < 0.01). Similar deflections were seen at oxygenation changes in Hb. The T1 changes suggests that the oxygen content has been changed in the specimen. The shortening of transverse relaxation times in T2 and T2*-mapping after deoxygenation in Mb specimens are highly indicative of a BOLD-like effect.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie

UniBE Contributor:

Günsch, Dominik; Jung, Bernd; Gulac, Patrik; Segiser, Adrian; Henning Longnus, Sarah and Fischer, Kady Anne

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Springer Nature

Language:

English

Submitter:

Maria de Fatima Henriques Bernardo

Date Deposited:

21 Jun 2021 14:48

Last Modified:

27 Jun 2021 03:07

Publisher DOI:

10.1038/s41598-021-90908-x

PubMed ID:

34075096

BORIS DOI:

10.48350/156683

URI:

https://boris.unibe.ch/id/eprint/156683

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