Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling

Halm, Sebastian; Haberthür, David; Eppler, Elisabeth; Djonov, Valentin Georgiev; Arnold, Andreas Michael (2021). Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling. Journal of Otolaryngology - Head and Neck Surgery, 50(1), p. 33. BioMed Central 10.1186/s40463-021-00522-0

[img]
Preview
Text
s40463-021-00522-0.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (8MB) | Preview

Introduction
This pilot study explores whether a human Thiel-embalmed temporal bone is suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and whether solid iodine-staining improves visualization and facilitates segmentation of middle ear structures.

Methods
A temporal bone was used to verify the accuracy of the imaging by first digitally measuring the stapes on the tomography images and then physically under the microscope after removal from the temporal bone. All measurements were compared with literature values.

The contralateral temporal bone was used to evaluate segmentation and three-dimensional (3D) modeling after iodine staining and micro-CT scanning.

Results
The digital and physical stapes measurements differed by 0.01–0.17 mm or 1–19%, respectively, but correlated well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor of 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan.

Conclusions
Micro-CT imaging of a Thiel-embalmed temporal bone accurately represented the entire anatomy. Iodine staining considerably increased the contrast of soft tissues, simplified segmentation and enabled detailed 3D modeling of the middle ear.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Halm, Sebastian; Haberthür, David; Eppler, Elisabeth; Djonov, Valentin Georgiev and Arnold, Andreas Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1916-0216

Publisher:

BioMed Central

Language:

English

Submitter:

David Christian Haberthür

Date Deposited:

28 Jun 2021 16:05

Last Modified:

04 Jul 2021 03:08

Publisher DOI:

10.1186/s40463-021-00522-0

PubMed ID:

34078459

BORIS DOI:

10.48350/156813

URI:

https://boris.unibe.ch/id/eprint/156813

Actions (login required)

Edit item Edit item
Provide Feedback