Characterization of Third-Generation Cephalosporin-Resistant Escherichia coli Isolated from Pigs in Cuba Using Next-Generation Sequencing.

Hernandez-Fillor, Rosa Elena; Brilhante, Michael; Marrero-Moreno, Carelia Martha; Baez, Michel; Espinosa, Ivette; Perreten, Vincent (2021). Characterization of Third-Generation Cephalosporin-Resistant Escherichia coli Isolated from Pigs in Cuba Using Next-Generation Sequencing. Microbial drug resistance, 27(7), pp. 1003-1010. Mary Ann Liebert 10.1089/mdr.2020.0174

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Resistance to third-generation cephalosporins (3GC) in Escherichia coli has been reported worldwide from humans and animals, but the situation in Cuba is still poorly understood. This study aimed to gain new insights into the phenotypic and genotypic characteristics of third-generation cephalosporin-resistant (3GC-R) E. coli isolated from pigs in Cuba. Rectal swabs from 215 healthy pigs were taken from different municipalities in the western region of Cuba and spread on MacConkey agar supplemented with cefotaxime and ceftazidime. Ninety-six isolates were identified as 3GC-R E. coli and 87.5% of them were resistant to at least three antibiotic classes as determined by the measurement of the minimum inhibitory concentration (MIC) of 14 antibiotics. Twenty-seven different isolates were selected for Illumina next-generation sequencing, and subsequent in silico analysis was performed for the detection of antibiotic resistance and virulence genes, plasmid incompatibility (Inc) groups, multilocus sequence typing (MLST), and core genome MLST (cgMLST). The sequenced isolates contained extended-spectrum β-lactamase genes blaCTX-M-32 (n = 17), blaCTX-M-15 (n = 5), and blaCTX-M-55 (n = 4) as well as with pAmpC gene blaCMY-2 (n = 2). They also harbored genes for resistance to other clinically important classes of antibiotics, as well as several diverse virulence factors. The 3GC-R E. coli were genetically highly diverse, belonging to 16 different sequence types. IncX1 was the most frequent Inc group. The presence of 3GC-R E. coli in pigs from Cuba containing several different antibiotic resistance mechanisms emphasizes the need for surveillance programs and the establishment of strategies for the prudent use of antibiotics in food-producing animals.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Hernandez Fillor, Rosa Elena; Quinta Brilhante, Michael and Perreten, Vincent

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1076-6294

Publisher:

Mary Ann Liebert

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

05 Aug 2021 11:48

Last Modified:

05 Dec 2022 15:52

Publisher DOI:

10.1089/mdr.2020.0174

PubMed ID:

33470893

Uncontrolled Keywords:

E. coli ESBLs NGS WGS cgMLST food-producing animals genotyping multidrug resistance

BORIS DOI:

10.48350/157823

URI:

https://boris.unibe.ch/id/eprint/157823

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