Korkmaz, Halil Ibrahim; Ulrich, Magda M W; Wieringen, Wessel N Van; Doǧan, Hatice; Vlig, Marcel; Emmens, Reindert W; Meyer, Klaas W; Sinnige, Paul; Zeerleder, Sacha; Wouters, Diana; Ham, Marieke S Van; Zuijlen, Paul P M Van; Krijnen, Paul A J; Niessen, Hans W M (2021). C1 Inhibitor Administration Reduces Local Inflammation and Capillary Leakage, Without Affecting Long-term Wound Healing Parameters, in a Pig Burn Wound Model. Anti-inflammatory & anti-allergy agents in medicinal chemistry, 20(2), pp. 150-160. Bentham Science 10.2174/1871523019666200702101513
Full text not available from this repository.BACKGROUND
Burns induce a boost in local and systemic complement levels as well as immune cell infiltration in the burn wound, which may negatively affect wound healing.
OBJECTIVE
In this study, the effects of long-term treatment with complement inhibitor C1 esterase inhibitor (C1inh) on post-burn inflammation and wound healing parameters were analyzed in time up to 60 days post-burn.
METHODS
Burned pigs were treated either with or without C1inh up to 15 days post-burn. Burn wound biopsies and blood were collected at different time points up to 60 days post-burn. Thereafter, complement in blood as well as complement and immune cells in the wound, capillary leakage, necrosis, reepithelialization and wound contraction were quantified.
RESULTS
No significant differences in complement C3 blood levels were observed at any time point between C1inh-treated and control pigs. In the wound, complement C4 levels were significantly lower in the C1inh group than in controls at day 3-6 and 21-30 post-burn. Similarly, C3 levels, neutrophil and macrophage infiltration in the wound were, although not statistically significant, reduced in C1inh-treated pigs at day 9-14 post-burn. No differences in lymphocyte infiltration in the wound were found between C1inh and control pigs. C1inh-treated pigs also showed reduced capillary leakage. Despite these effects, no significant differences in the long-term wound healing parameters necrosis, reepithelialization and wound contraction were observed between C1inh and control pigs.
CONCLUSION
In pigs, 15 days of C1inh treatment after burn, leads to a reduction in local inflammation and capillary leakage in the burn wound without affecting long-term wound healing parameters.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory |
UniBE Contributor: |
Zeerleder, Sacha Sergio |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1875-614X |
Publisher: |
Bentham Science |
Language: |
English |
Submitter: |
Pierrette Durand Lüthi |
Date Deposited: |
11 Nov 2021 17:22 |
Last Modified: |
05 Dec 2022 15:53 |
Publisher DOI: |
10.2174/1871523019666200702101513 |
PubMed ID: |
32614753 |
Uncontrolled Keywords: |
C1 inhibitor burn wound capillary leakage complement inflammation pig burn wound model wound healing |
URI: |
https://boris.unibe.ch/id/eprint/159613 |
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