Sisakian, H S; Mkhitaryan, A G; Sahakyan, V G; Kocharyan, A S; Asatryan, B A (2019). [Morphological and Immunohystochemical Characteristics of Aspirated Thrombi in Patients with ST-Elevation Myocardial Infarction]. Terapevticheskii arkhiv, 91(9), pp. 21-25. CONSILIUM MEDICUM LLC 10.26442/00403660.2019.09.000199
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The aim of study was to evaluate the morphological and immunohystochemical characteristics of aspirated thrombi in patients with ST-elevation myocardial infarction undergoing precutaneous coronary intervention to find the possible platelet activation and inflammatory cell involvement in coronary thrombi.
MATERIALS AND METHODS
Thrombi collected from 67 patients with ST-elevation myocardial infarction were studied. Macroscopic and immunohystochemical analyses were performed to reveal the cellular components of thrombi. The peroxidase - antiperoxidase immunohystochemical method was applied with monoclonal antibodies to CD4+, CD8+, CD15+, CD63+ and CD105+ using diaminobenzidine agent as a visualization agent to investigate the potential involvement of different cellular subpopulations.
RESULTS
The macroscopic examination revealed friable thrombi with apparent laminations (lines of Zahn). Microscopic analysis of thrombi revealed a loose meshwork of fibrin fibers with compact accumulation of activated platelets. The platelets were surrounded by neutrophilic granulocytes.The immunohystochemical analysis confirmed that cell aggregates are consisting of CD 63+ activated platelets and leucocytes. The granulocytes also tested positive for CD15 antigen. The immunohystochemical analysis revealed the presence of CD8+ cytotoxic T-lymphocytes and CD4+ helper T-lymphocytes. No expression of iNOS, CD105 and VEGF was found in the analyzed thrombi.
CONCLUSIONS
The hystopathological evaluation of thrombi in patients with acute ST-elevation myocardial infarction revealed the presence of activated CD63+ platelets and CD15+ neutrophilic granulocytes. Activated platelets and neutrophils may play a role in thromboinflammatory activation course leading to destabilization of atherosclerotic plaque and development of acute thrombosis in patients with ST-elevation acute myocardial infarction.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology |
UniBE Contributor: |
Asatryan, Babken |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0040-3660 |
Publisher: |
CONSILIUM MEDICUM LLC |
Language: |
English |
Submitter: |
Babken Asatryan |
Date Deposited: |
03 Dec 2021 12:20 |
Last Modified: |
05 Dec 2022 15:54 |
Publisher DOI: |
10.26442/00403660.2019.09.000199 |
PubMed ID: |
32598810 |
Uncontrolled Keywords: |
activated platelets coronary thrombosis immunohistochemistry myocardial infarction thrombectomy |
BORIS DOI: |
10.48350/160951 |
URI: |
https://boris.unibe.ch/id/eprint/160951 |
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