Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface.

Taiqoui Pelá, Vinícius; Buzalaf, Marília Afonso Rabelo; Niemeyer, Samira; Baumann, Tommy; Henrique-Silva, Flávio; Toyama, Danyelle; Crusca, Edson; Marchetto, Reinaldo; Lussi, Adrian; Saads Carvalho, Thiago (2021). Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface. Journal of dentistry, 107, p. 103612. Elsevier Science 10.1016/j.jdent.2021.103612

[img] Text
1-s2.0-S0300571221000336-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (4MB) | Request a copy

OBJECTIVE

This study investigated the mechanism of action of different proteins/peptides (separately or in combination), focusing on how they act directly on the native enamel surface and on modifying the salivary pellicle.

METHODS

A total of 170 native human enamel specimens were prepared and submitted to different treatments (2 h; 37 °C): with deionized water, CaneCPI-5, Hemoglobin, Statherin, or a combination of all three proteins/peptides. The groups were subdivided into treatment acting on the enamel surface (NoP - absence of salivary pellicle), and treatment modifying the salivary pellicle (P). Treatment was made (2 h; 37 °C) in all specimens, and later, for P, the specimens were incubated in human saliva (2 h; 37 °C). In both cases, the specimens were immersed in 1% citric acid (pH 3.6; 2 min; 25 °C). Calcium released from enamel (CaR) and its relative surface reflection intensity (%SRI) was measured after 5 cycles. Between-group differences were verified with two-way ANOVA, with "presence of pellicle" and "treatment" as factors (α = 0.05).

RESULTS

The presence of pellicle provided better protection regarding %SRI (p < 0.01), but not regarding CaR (p = 0.201). In relation to treatment, when compared to the control group, all proteins/peptides provided significantly better protection (p < 0.01 for %SRI and Car). The combination of all three proteins/peptides demonstrated the best protective effect (p < 0.01 for %SRI).

CONCLUSION

Depending on the protein or peptide, its erosion-inhibiting effect derives from their interaction with the enamel surface or from modifying the pellicle, so a combination of proteins and peptides provides the best protection.

CLINICAL SIGNIFICANCE

The present study opens a new direction for a possible treatment with a combination of proteins for native human enamel, which can act directly on the enamel surface as well on the modification of the salivary pellicle, for the prevention of dental erosion.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine > Restorative Dentistry, Research

UniBE Contributor:

Taiqoui Pelá, Vinícius; Niemeyer, Samira Helena; Baumann, Tommy and Saads Carvalho, Thiago

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0300-5712

Publisher:

Elsevier Science

Language:

English

Submitter:

Tommy Baumann

Date Deposited:

05 Jan 2022 16:16

Last Modified:

09 Jan 2022 01:51

Publisher DOI:

10.1016/j.jdent.2021.103612

PubMed ID:

33621617

Uncontrolled Keywords:

Acquired pellicle Dental erosion Enamel Pellicle modification Protein saliva

BORIS DOI:

10.48350/162163

URI:

https://boris.unibe.ch/id/eprint/162163

Actions (login required)

Edit item Edit item
Provide Feedback