Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface.

Taiqoui Pelá, Vinícius; Buzalaf, Marília Afonso Rabelo; Niemeyer, Samira; Baumann, Tommy; Henrique-Silva, Flávio; Toyama, Danyelle; Crusca, Edson; Marchetto, Reinaldo; Lussi, Adrian; Saads Carvalho, Thiago (2021). Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface. Journal of dentistry, 107, p. 103612. Elsevier Science 10.1016/j.jdent.2021.103612

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This study investigated the mechanism of action of different proteins/peptides (separately or in combination), focusing on how they act directly on the native enamel surface and on modifying the salivary pellicle.


A total of 170 native human enamel specimens were prepared and submitted to different treatments (2 h; 37 °C): with deionized water, CaneCPI-5, Hemoglobin, Statherin, or a combination of all three proteins/peptides. The groups were subdivided into treatment acting on the enamel surface (NoP - absence of salivary pellicle), and treatment modifying the salivary pellicle (P). Treatment was made (2 h; 37 °C) in all specimens, and later, for P, the specimens were incubated in human saliva (2 h; 37 °C). In both cases, the specimens were immersed in 1% citric acid (pH 3.6; 2 min; 25 °C). Calcium released from enamel (CaR) and its relative surface reflection intensity (%SRI) was measured after 5 cycles. Between-group differences were verified with two-way ANOVA, with "presence of pellicle" and "treatment" as factors (α = 0.05).


The presence of pellicle provided better protection regarding %SRI (p < 0.01), but not regarding CaR (p = 0.201). In relation to treatment, when compared to the control group, all proteins/peptides provided significantly better protection (p < 0.01 for %SRI and Car). The combination of all three proteins/peptides demonstrated the best protective effect (p < 0.01 for %SRI).


Depending on the protein or peptide, its erosion-inhibiting effect derives from their interaction with the enamel surface or from modifying the pellicle, so a combination of proteins and peptides provides the best protection.


The present study opens a new direction for a possible treatment with a combination of proteins for native human enamel, which can act directly on the enamel surface as well on the modification of the salivary pellicle, for the prevention of dental erosion.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine > Restorative Dentistry, Research

UniBE Contributor:

Taiqoui Pelá, Vinícius, Niemeyer, Samira Helena, Baumann, Tommy, Saads Carvalho, Thiago


600 Technology > 610 Medicine & health




Elsevier Science




Tommy Baumann

Date Deposited:

05 Jan 2022 16:16

Last Modified:

05 Dec 2022 15:56

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Acquired pellicle Dental erosion Enamel Pellicle modification Protein saliva




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